ARG30010

Neuronal Cytoskeletal Antibody Duo (NF-L, TUBBIII)

Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG52348 anti-Neurofilament NF-L antibody [DA2] Mouse mAb Ms, Rat FACS, ICC/IF, IHC-P, IHC-Fr, WB 50 μl
ARG62683 anti-beta III Tubulin antibody [TU-20] Mouse mAb Bb, Bov, Broad, Carp, Chk, Hm, Hu, Ms, Pig, Rat FACS, ICC/IF, IHC-P, WB 50 μg

Overview

Product Description

TuJ1/TUBBIII, a class III member of the beta tubulin protein family, is found in the cell bodies, dendrites, axons, and axonal terminations of immature neurons as a marker for immature neurons. NF-L, neurofilament light or low polypeptide, a 68-70 kDa protein, is a member of neurofilaments. Neurofilaments are a major component of the neuronal cytoskeleton, and are believed to function primarily to provide structural support for the axon and to regulate axon diameter. NF-L is as a marker for differentiated post-mitotic neuronal cells.

ARG30010 Neuronal cytoskeletal Duos (NF-L, TUBBIII), including antibodies react two frequently used neuronal cytoskeletal target, NF-L and TUBBIII. This antibody duo not only provides neuronal cytoskeletal marker, but also inclueds neuron markers for differentiated neuronal and immature neuron which will be useful in neurogenesis, neural stem cell and neuronal disease studies.

Properties

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Research Area Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody

Images (6) Click the Picture to Zoom In

  • ARG62683 anti-beta III Tubulin antibody [TU-20] WB image

    Western blot: 30 µg of 1) HeLa (negative control), 2) Mouse brain, and 3) Rat brain lysate stained with ARG62683 anti-beta III Tubulin antibody [TU-20] at 1:1000 dilution.

  • ARG52348 anti-Neurofilament NF-L antibody [DA2] WB image

    Western blot: 30 µg of Mouse brain lysate stained with ARG52348 anti-Neurofilament NF-L antibody [DA2] at 1:1000 dilution.

  • ARG52348 anti-Neurofilament NF-L antibody [DA2] WB image

    Western blot: rat cortex lysate stained with ARG52348 anti-Neurofilament NF-L antibody [DA2] showing specific immunolableing of the ~ 68k NF-L protein.

  • Monoclonal antibody clone TU-20 ICC/IF image

    Immunofluorescence: Neuro2a mouse neuroblastoma cell stained with clone TU-20 (green)
    Cell nuclei was stained with DAPI (blue).

  • Monoclonal antibody clone TU-20 ICC/IF image

    Immunofluorescence: P-19 mouse embryonal carcinoma cells stimulated to neuronal differentiation by retinoic acid stained with clone TU-20 (red)
    Cell nuclei was stained with DAPI (blue).

  • Monoclonal antibody clone TU-20 ICC/IF image

    Immunofluorescence: P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid co-stained with stained with clone TU-20 (red) and anti-beta-tubulin (green)
    Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).