ARG30254

Phospho NFkB p65 Antibody Duo (Total, pT435)

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Immune System antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody
publication_link Publication5

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG51516 anti-NFkB p65 phospho (Thr435) antibody Rabbit pAb Hu, Ms, Rat ICC/IF, IHC-P, WB 50 μl
ARG65677 anti-NFkB p65 antibody Mouse mAb Hu, Ms, Rat ICC/IF, IHC-P, WB 50 μg

Overview

Product Description Nuclear factor kappa B (NF-κB) is a protein complex that controls large number of genes responsible for the regulation of apoptosis, inflammation, tumorigenesis and a variety of immune responses. NF-kB is sensitive and responsive to stimuli such as cytokines, stress, UV, low oxygen, bacterial or viral infections. In most cell types, NF-κB subunits exist as hetero- or homodimers bound to the inhibitory IκB proteins, which retain the complex mainly in the cytoplasm. Once activated, IκB proteins are phosphorylated by IKKα and IKKβ, leading to the degradation of IκB proteins and nuclear translocation of NF-κB proteins. Post-translation modification such as phosphorylation of NF-κB p65 at Thr435 site leads to transcription activity and NF-κB subunit transactivation.
  O’Shea and Perkins. Biochem J. 2010. 426: 345-354
Hayden and Ghosh. (2012) Genes and Dev 26: 203-234 Baeuerle and Henkel. (1994) Ann Rev Immunol 12: 141-179

Related news:
Exploring Antiviral Immune Response
Target Name NFkB p65
Alternate Names Phospho NFkB p65 antibody; NFkB p65 phospho (Thr435) antibody; NFkB p65 antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Phospho NFkB p65 Antibody Duo (Total, pT435)
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Immune System antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody

Images (5) Click the Picture to Zoom In

  • ARG65677 anti-NFkB p65 antibody WB image

    Western blot: 30 µg of HeLa cell lysate stained with ARG65677 anti-NFkB p65 antibody at 1:500 dilution.

  • ARG51516 anti-NFkB p65 phospho (Thr435) antibody WB image

    Western Blot: extracts from HeLa cells untreated or treated with TNFa+CA stained with anti-NFkB p65 (phospho Thr435) antibody ARG51516.

  • ARG51516 anti-NFkB p65 phospho (Thr435) antibody IHC-P image

    Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-NFkB p65 (phospho Thr435) antibody ARG51516).

  • ARG51516 anti-NFkB p65 phospho (Thr435) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with anti-NFkB p65 (phospho Thr435) antibody ARG51516, Red).

  • ARG65677 anti-NFkB antibody IHC image

    Immunohistochemistry: Mouse hippocampus tissue stained with ARG65677 anti-NFkB antibody at 1:200 dilution.

Specific References

Downregulation of lncRNA NEAT1 alleviates sepsis-induced acute kidney injury

ARG65677: WB / Human

Yuhua Zhou et al.
Central European Journal of Immunology,  (2022)

publication_link

 

hr_line

OC-STAMP Overexpression Drives Lung Alveolar Epithelial Cell Type II Senescence in Silicosis.

ARG51516: WB / Rat

Tian Li et al.
Oxidative Medicine and Cellular Longevity.,  (2021)

publication_link

 

hr_line

Inhibition of inflammatory response by α-lipoic acid in autoimmune thyroiditis mice.

ARG65677: WB / Mouse

Mayinu Yusufu et al.
Basic & Clinical Medicine.,  (2021)

publication_link

 

hr_line

The fucoidan from sea cucumber Apostichopus japonicus attenuates lipopolysaccharide-challenged liver injury in C57BL/6J mice.

WB / Mouse

Yin Jiayu et al.
Journal of Functional Foods.,  (2019)

publication_link

 

hr_line

Activation of GABAB Receptor Suppresses Diabetic Neuropathic Pain through Toll-Like Receptor 4 Signaling Pathway in the Spinal Dorsal Horn.

ARG65677: WB, ICC/IF / Rat

Liu Peng et al.
Mediators of inflammation.,  (2018)

publication_link

 

hr_line