ARG30302

T-cell infiltration Antibody Duo

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG65859 anti-CD3 antibody [SQab1713] Rabbit mAb Hu FACS, ICC/IF, IHC-P, IP, WB 50 μl
ARG65860 anti-CD4 antibody [SQab1714] Rabbit mAb Hu FACS, IHC-P, IP, WB 50 μl

Overview

Product Description T-cells have the ability to leave the bloodstream and migrate into and attack tumor cells. Evidences show that enhanced T-cell infiltration in tumor tissue result to increased survival. T-cell infiltration is critical for examining the effect of cancer immunotherapy. arigo’s T-cell Infiltration Antibody Duo offers two quality antibodies against CD3 and CD4. It is the best solution for labeling infiltrating T-cells in tissue.
Target Name T-cell infiltration
Full Name Antibody Duo for T-cell infiltration
Alternate Names T-cell infiltration antibody; CD3 antibody; CD4 antibody

Properties

Note For laboratory research only, not for drug, diagnostic or other use.

Images (10) Click the Picture to Zoom In

  • ARG65859 anti-CD3 antibody [SQab1713] WB image

    Western blot: 30 µg of Molt4 cell lysate stained with ARG65859 anti-CD3 antibody [SQab1713] at 1:500 dilution.

  • ARG65860 anti-CD4 antibody [SQab1714] WB image

    Western blot: 30 µg of Jurkat and Molt4 cell lysates stained with ARG65860 anti-CD4 antibody [SQab1714] at 1:500 dilution.

  • ARG65859 anti-CD3 antibody [SQab1713] ICC/IF image

    Immunofluorescence: Jurkat cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% Goat serum for half an hour at room temperature. Samples were stained with ARG65859 anti-CD3 antibody [SQab1713] (green) at 1:50 and 4°C. DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody.

  • ARG65859 anti-CD3 antibody [SQab1713] FACS image

    Flow Cytometry: Jurkat cells were fixed with 4% paraformaldehyde for 10 min. The cells were then stained with ARG65859 anti-CD3 antibody [SQab1713] (blue) at 1:1000 dilution in 1x PBS/1% BSA for 30 min at room temperture, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (red) was used as a control.

  • ARG65860 anti-CD4 antibody [SQab1714] FACS image

    Flow Cytometry: Jurkat cells were fixed with 4% paraformaldehyde for 10 min. The cells were then stained with ARG65860 anti-CD4 antibody [SQab1714] (blue) at 1:50 dilution in 1x PBS/1% BSA for 30 min at room temperture, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (red) was used as a control.

  • ARG65859 anti-CD3 antibody [SQab1713] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human tonsil tissue stained with ARG65859 anti-CD3 antibody [SQab1713] at 1:200 dilution. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).

  • ARG65860 anti-CD4 antibody [SQab1714] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human tonsil tissue stained with ARG65860 anti-CD4 antibody [SQab1714] at 1:2000 dilution. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).

  • ARG65859 anti-CD3 antibody [SQab1713] IP image

    Immunoprecipitation: 0.4 mg of Molt-4 whole cell lysate was immunoprecipitated (1:15 dilution) and stained with ARG65859 anti-CD3 antibody [SQab1713].

    Lane 1: Immunoprecipitation in Molt-4 whole cell lysate
    Lane 2: Rabbit IgG instead of Primary Ab in Molt-4 whole cell lysate
    Lane 3: Molt-4 whole cell lysate, 10 µg (input)

  • ARG65860 anti-CD4 antibody [SQab1714] IP image

    Immunoprecipitation: 0.4 mg of Molt-4 whole cell lysate was immunoprecipitated (1:50 dilution) and stained with ARG65860 anti-CD4 antibody [SQab1714].

    Lane 1: Immunoprecipitation in Molt-4 whole cell lysate
    Lane 2: Rabbit IgG instead of Primary Ab in Molt-4 whole cell lysate
    Lane 3: Molt-4 whole cell lysate, 10 µg (input)

  • ARG65859 anti-CD3 antibody [SQab1713] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human colon tissue stained with ARG65859 anti-CD3 antibody [SQab1713] at 1:200 dilution. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).