anti-CD4 antibody [SQab1714]
anti-CD4 antibody [SQab1714] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Developmental Biology antibody; Immune System antibody; Regulatory T cells Study antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody
Recombinant Rabbit Monoclonal antibody [SQab1714] recognizes CD4
|Tested Application||FACS, IHC-P, IP, WB|
|Immunogen||Synthetic peptide around aa. 200-400 of Human CD4.|
|Full Name||CD4 molecule|
|Alternate Names||CD4mut; CD antigen CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3|
|Application Note||IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Calculated MW||51 kDa|
|Purification||Purification with Protein A.|
|Buffer||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.|
|Preservative||0.01% Sodium azide|
|Stabilizer||40% Glycerol and 0.05% BSA|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||CD4 molecule|
|Background||CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]|
|Function||CD4 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. [UniProt]|
|Highlight||Related Antibody Duos and Panels:
ARG30302 T-cell infiltration Antibody Duo
ARG30334 Tumor-infiltrating Lymphocyte Antibody Panel
CD4 antibodies; CD4 ELISA Kits; CD4 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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|Research Area||Developmental Biology antibody; Immune System antibody; Regulatory T cells Study antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody|
|PTM||Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.|
Images (4) Click the Picture to Zoom In
ARG65860 anti-CD4 antibody [SQab1714] WB image
Western blot: 30 µg of Jurkat and Molt4 cell lysates stained with ARG65860 anti-CD4 antibody [SQab1714] at 1:500 dilution.
ARG65860 anti-CD4 antibody [SQab1714] FACS image
Flow Cytometry: Jurkat cells were fixed with 4% paraformaldehyde for 10 min. The cells were then stained with ARG65860 anti-CD4 antibody [SQab1714] (blue) at 1:50 dilution in 1x PBS/1% BSA for 30 min at room temperture, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (red) was used as a control.
ARG65860 anti-CD4 antibody [SQab1714] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human tonsil tissue stained with ARG65860 anti-CD4 antibody [SQab1714] at 1:2000 dilution. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
ARG65860 anti-CD4 antibody [SQab1714] IP image
Immunoprecipitation: 0.4 mg of Molt-4 whole cell lysate was immunoprecipitated (1:50 dilution) and stained with ARG65860 anti-CD4 antibody [SQab1714].
Lane 1: Immunoprecipitation in Molt-4 whole cell lysate
Lane 2: Rabbit IgG instead of Primary Ab in Molt-4 whole cell lysate
Lane 3: Molt-4 whole cell lysate, 10 µg (input)