anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1]

anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Other

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody; Neuroscience antibody
publication_link Publication2


Product Description

Mouse Monoclonal antibody [MoBu-1] recognizes 5-bromodeoxyuridine / BrdU

Tested Reactivity Other
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P
Specificity The clone MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. MoBu-1 is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. It reacts also specifically with 5-bromouridine (BrU). The clone does not cross-react to EdU (PMID: 23272138).
Host Mouse
Clonality Monoclonal
Clone MoBu-1
Isotype IgG1
Target Name 5-bromodeoxyuridine / BrdU
Immunogen 5-bromodeoxyuridine conjugated with hemocyanine.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 2 µg/ml
ICC/IF2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Background Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.
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Research Area Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody; Neuroscience antibody

Images (2) Click the Picture to Zoom In

  • ARG63121 anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1] FACS image

    Flow Cytometry: 5-bromodeoxyuridin (BrdU) incorporated CEM cells stained with ARG63121 anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1], followed by incubation with FITC-labelled secondary antibody.

  • ARG63121 anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1] IHC-P image

    Immunohistochemistry: Bromodeoxyuridine-labeled cells (chick embryo; paraffin-embedded sections) stained with ARG63121 anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1].

Specific References

Vascular Endothelial Cell-derived Exosomes Protect Neural Stem Cells Against Ischemia/reperfusion Injury.

IHC-Fr / Other

Zhou Shaoting et al.
Neuroscience.,  (2020)




Activation of AMPK pathway compromises Rab11 downregulation-mediated inhibition of Schwann cell proliferation in a Glut1 and Glut3-dependent manner.

ICC/IF / Other

Li Fan et al.
Neurosci Lett.,  (2020)