ARG10598

anti-ABCB11 antibody

anti-ABCB11 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot,Flow cytometry and Human,Mouse,Rat

publication_link Publication2

Overview

Product Description

Rabbit Polyclonal antibody recognizes ABCB11

Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Target Name ABCB11
Antigen Species Human
Immunogen aa. KYGDNTKEIPMERVIAAAKQAQLHD of Human ABCB11.
Conjugation Un-conjugated
Alternate Names SPGP; ABC16; BRIC2; PGY4; Bile salt export pump; PFIC2; PFIC-2; ATP-binding cassette sub-family B member 11; BSEP

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
IHC-P0.5 - 2 µg/ml
WB0.1 - 0.5 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil the tissue sections in 10 mM Citrate buffer (pH 6.0) for 20 min and allow to cool prior to staining.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.025% Sodium azide and 2.5% BSA.
Preservative 0.025% Sodium azide
Stabilizer 2.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 27413 Mouse ABCB11

GeneID: 83569 Rat ABCB11

GeneID: 8647 Human ABCB11

Gene Symbol ABCB11
Gene Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 11
Background The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. [provided by RefSeq, Jul 2008]
Function Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. [UniProt]
Calculated MW 146 kDa

Images (6) Click the Picture to Zoom In

  • ARG10598 anti-ABCB11 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and Paraffin-embedded Human liver cancer stained with ARG10598 anti-ABCB11 antibody.

  • ARG10598 anti-ABCB11 antibody IHC image

    Immunohistochemistry: Mouse liver tissue stained with ARG10598 anti-ABCB11 antibody (red). DAPI (blue) for nuclear staining.

  • ARG10598 anti-ABCB11 antibody WB image

    Western blot: 1) Rat liver, 2) Mouse liver and 3) Human SMMC lysate stained with ARG10598 anti-ABCB11 antibody.

  • ARG10598 anti-ABCB11 antibody FACS image

    Flow Cytometry: A431 cells were blocked with goat sera and stained with ARG10598 anti-ABCB11 antibody at 1 µg/10^6 cells (blue); Cells alone (red); Isotype control (green).

  • ARG10598 anti-ABCB11 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and Paraffin-embedded Mouse liver stained with ARG10598 anti-ABCB11 antibody.

  • ARG10598 anti-ABCB11 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and Paraffin-embedded Rat liver stained with ARG10598 anti-ABCB11 antibody.

Specific References

The protective effects and mechanism of Alismatis Rhizoma extracts against senecionine-induced acute liver injury in mice.

WB / Mouse

Kai-yuan Jiang et al.
Acta Pharmaceutica Sinica.,  (2021)

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Vertical sleeve gastrectomy confers metabolic improvements by reducing intestinal bile acids and lipid absorption in mice.

WB / Mouse

Lili Ding et al.
Proc Natl Acad Sci USA.,  (2021)

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