ARG10598
anti-ABCB11 antibody
anti-ABCB11 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Publication2
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ABCB11 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ABCB11 |
| Antigen Species | Human |
| Immunogen | aa. KYGDNTKEIPMERVIAAAKQAQLHD of Human ABCB11. |
| Conjugation | Un-conjugated |
| Alternate Names | SPGP; ABC16; BRIC2; PGY4; Bile salt export pump; PFIC2; PFIC-2; ATP-binding cassette sub-family B member 11; BSEP |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil the tissue sections in 10 mM Citrate buffer (pH 6.0) for 20 min and allow to cool prior to staining. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.025% Sodium azide and 2.5% BSA. |
| Preservative | 0.025% Sodium azide |
| Stabilizer | 2.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ABCB11 |
| Gene Full Name | ATP-binding cassette, sub-family B (MDR/TAP), member 11 |
| Background | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. [provided by RefSeq, Jul 2008] |
| Function | Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. [UniProt] |
| Calculated MW | 146 kDa |
Images (7) Click the Picture to Zoom In
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ARG10598 anti-ABCB11 antibody WB image
Western blot: Mouse liver stained with ARG10598 anti-ABCB11 antibody.
From Ding L et al. Proc Natl Acad Sci USA- (2021), doi: 10.1073/pnas.2019388118, Fig. 2. B.
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ARG10598 anti-ABCB11 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and Paraffin-embedded Human liver cancer stained with ARG10598 anti-ABCB11 antibody.
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ARG10598 anti-ABCB11 antibody IHC image
Immunohistochemistry: Mouse liver tissue stained with ARG10598 anti-ABCB11 antibody (red). DAPI (blue) for nuclear staining.
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ARG10598 anti-ABCB11 antibody WB image
Western blot: 1) Rat liver, 2) Mouse liver and 3) Human SMMC lysate stained with ARG10598 anti-ABCB11 antibody.
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ARG10598 anti-ABCB11 antibody FACS image
Flow Cytometry: A431 cells were blocked with goat sera and stained with ARG10598 anti-ABCB11 antibody at 1 µg/10^6 cells (blue); Cells alone (red); Isotype control (green).
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ARG10598 anti-ABCB11 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and Paraffin-embedded Mouse liver stained with ARG10598 anti-ABCB11 antibody.
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ARG10598 anti-ABCB11 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and Paraffin-embedded Rat liver stained with ARG10598 anti-ABCB11 antibody.
Specific References
Vertical sleeve gastrectomy confers metabolic improvements by reducing intestinal bile acids and lipid absorption in mice.
WB / Mouse
The protective effects and mechanism of Alismatis Rhizoma extracts against senecionine-induced acute liver injury in mice.
WB / Mouse
