ARG54808

anti-ALG14 antibody

anti-ALG14 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human

Controls and Markers antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes ALG14
Tested Reactivity Hu
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ALG14
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 67-93 (Center) of Human ALG14.
Conjugation Un-conjugated
Alternate Names UDP-N-acetylglucosamine transferase subunit ALG14 homolog; CMS15

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control NCI-H460

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 199857 Human ALG14

Swiss-port # Q96F25 Human UDP-N-acetylglucosamine transferase subunit ALG14 homolog

Gene Symbol ALG14
Gene Full Name ALG14, UDP-N-acetylglucosaminyltransferase subunit
Background This gene is a member of the glycosyltransferase 1 family. The encoded protein and ALG13 are thought to be subunits of UDP-GlcNAc transferase, which catalyzes the first two committed steps in endoplasmic reticulum N-linked glycosylation. Mutations in this gene have been linked to congenital myasthenic syndrome (CMSWTA). Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]
Function May be involved in protein N-glycosylation. May play a role in the second step of the dolichol-linked oligosaccharide pathway. May anchor the catalytic subunit ALG13 to the ER. [UniProt]
Cellular Localization Endoplasmic reticulum membrane; Single-pass membrane protein
Research Area Controls and Markers antibody
Calculated MW 24 kDa

Images (3) Click the Picture to Zoom In

  • ARG54808 anti-ALG14 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung carcinoma stained with ARG54808 anti-ALG14 antibody.

  • ARG54808 anti-ALG14 antibody WB image

    Western blot: 35 µg of NCI-H460 cell lysate stained with ARG54808 anti-ALG14 antibody.

  • ARG54808 anti-ALG14 antibody FACS image

    Flow Cytometry: NCI-H460 cells stained with ARG54808 anti-ALG14 antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.