ARG51678

anti-AMPK alpha 1 phospho (Thr183) + AMPK alpha 2 phospho (Thr172) antibody

anti-AMPK alpha 1 phospho (Thr183) + AMPK alpha 2 phospho (Thr172) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; AMPK-ACC pathway antibody
publication_link Publication4

Overview

Product Description Rabbit Polyclonal antibody recognizes AMPK alpha 1 phospho (Thr183) + AMPK alpha 2 phospho (Thr172)
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name AMPK alpha 1 + AMPK alpha 2
Antigen Species Human
Immunogen Phosphospecific peptide around Thr183 of Human AMPK alpha 1. (Also around Thr172 of Human AMPK alpha 2) (L-R-T(p)-S-C)
Conjugation Un-conjugated
Alternate Names AMPK alpha 1: AMPK; Acetyl-CoA carboxylase kinase; ACACA kinase; EC 2.7.11.26; EC 2.7.11.31; 5'-AMP-activated protein kinase catalytic subunit alpha-1; EC 2.7.11.27; HMGCR kinase; Tau-protein kinase PRKAA1; EC 2.7.11.1; Hydroxymethylglutaryl-CoA reductase kinase; AMPKa1; AMPK subunit alpha-1

AMPK alpha 2: AMPK; Acetyl-CoA carboxylase kinase; ACACA kinase; 5'-AMP-activated protein kinase catalytic subunit alpha-2; EC 2.7.11.31; EC 2.7.11.27; HMGCR kinase; PRKAA; AMPK2; EC 2.7.11.1; AMPK subunit alpha-2; AMPKa2; Hydroxymethylglutaryl-CoA reductase kinase

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 105787 Mouse PRKAA1

GeneID: 5562 Human PRKAA1

GeneID: 65248 Rat PRKAA1

Gene Symbol PRKAA1; PRKAA2
Gene Full Name protein kinase AMP-activated catalytic subunit alpha 1
protein kinase AMP-activated catalytic subunit alpha 2
Background AMPK alpha 1: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

AMPK alpha 2: The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008]
Function Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. [UniProt]
Cellular Localization AMPK alpha 1 and 2: Cytoplasm. Nucleus. Note=In response to stress, recruited by p53/TP53 to specific promoters. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30182 AMPK-ACC pathway Antibody Panel
Related products:
AMPK alpha antibodies; AMPK alpha Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; AMPK-ACC pathway antibody
Calculated MW 64 kDa
PTM AMPK alpha 1: Ubiquitinated.
Phosphorylated at Thr-183 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Also phosphorylated at Thr-183 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-183, but at a much lower level. Dephosphorylated by protein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK. Dephosphorylated by PPM1A and PPM1B. [UniProt]

AMPK alpha 2: Ubiquitinated.
Phosphorylated at Thr-172 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Also phosphorylated at Thr-172 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-172, but at much lower level. Dephosphorylated by protein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK. Dephosphorylated by PPM1A and PPM1B at Thr-172 (mediated by STK11/LKB1). [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG51678 anti-AMPK alpha 1 phospho (Thr183) + AMPK alpha 2 phospho (Thr172) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung carcinoma tissue stained with ARG51678 anti-AMPK alpha 1 phospho (Thr183) + AMPK alpha 2 phospho (Thr172) antibody.

  • ARG51678 anti-AMPK alpha 1 phospho (Thr183) + AMPK alpha 2 phospho (Thr172) antibody WB image

    Western blot: Extracts from C2C12 cells, treated with serum or calf intestinal phosphatase (CIP), stained with ARG51678 anti-AMPK alpha 1 phospho (Thr183) + AMPK alpha 2 phospho (Thr172) antibody.

Specific References

Amlexanox Enhances Temozolomide-Induced Antitumor Effects in Human Glioblastoma Cells by Inhibiting IKBKE and the Akt-mTOR Signaling Pathway

WB, IHC-P / Human

Jinbiao Xiong et al.
ACS Omega.,  (2021)

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Berberine improves ovulation and endometrial receptivity in polycystic ovary syndrome.

WB / Human

Zhi Wang et al.
Phytomedicine.,  (2021)

publication_link

 

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Pentadecanoic acid promotes basal and insulin-stimulated glucose uptake in C2C12 myotubes.

WB / Mouse

Wen-Cheng Fu et al.
Food & nutrition research.,  (2021)

publication_link

 

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Amlexanox Enhances Temozolomide-Induced Anti-Tumor Effects In Human Glioblastoma Cells By Inhibiting Ikbke And The Akt-mtor Signaling Pathway.

WB, IHC-P / Human

Jinbiao Xiong et al.
Preprint.,  (2020)

publication_link

 

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