ARG10696

anti-Arrestin 1 / S-Arrestin antibody [S128]

anti-Arrestin 1 / S-Arrestin antibody [S128] for Western blot,ICC/IF,IHC-Frozen sections and Pig,Bovine

Overview

Product Description Mouse Monoclonal antibody [S128] recognizes Arrestin 1 / S-Arrestin
Tested Reactivity Bov, Pig
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone S128
Isotype IgG1
Target Name Arrestin 1 / S-Arrestin
Antigen Species Bovine
Immunogen Recombinant Bovine Arrestin-1 with the first 20 aa of the C-terminus truncated.
Conjugation Un-conjugated
Full Name S-antigen; retina and pineal gland (arrestin)
Alternate Names RP47; 48 kDa protein; S-arrestin; Rod photoreceptor arrestin; S-AG; Retinal S-antigen

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000
IHC-Fr1:1000
WB1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 280922 Bovine SAG

GeneID: 397151 Pig SAG

Swiss-port # P08168 Bovine S-arrestin

Swiss-port # P79260 Pig S-arrestin

Gene Symbol SAG
Gene Full Name S-antigen; retina and pineal gland (arrestin)
Background Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. S-arrestin, also known as S-antigen, is a major soluble photoreceptor protein that is involved in desensitization of the photoactivated transduction cascade. It is expressed in the retina and the pineal gland and inhibits coupling of rhodopsin to transducin in vitro. Additionally, S-arrestin is highly antigenic, and is capable of inducing experimental autoimmune uveoretinitis. Mutations in this gene have been associated with Oguchi disease, a rare autosomal recessive form of night blindness. [provided by RefSeq, Jul 2008]
Function Arrestin is one of the major proteins of the ros (retinal rod outer segments); it binds to photoactivated-phosphorylated rhodopsin, thereby apparently preventing the transducin-mediated activation of phosphodiesterase. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG10696 anti-Arrestin 1 / S-Arrestin antibody [S128] IHC-Fr image

    Immunohistochemistry: Confocal image of a pig retina frozen section stained with ARG10696 anti-Arrestin 1 / S-Arrestin antibody [S128] (green). Visual arrestin is most abundant in the outer segments (OS) and inner surface of the outer nuclear layer (ONL), and can be used to identify components of rod photoreceptor cells. (Cone photoreceptors have a different arrestin isotype). Other retinal layers are inner segments (IS), outer plexiform layer (OPL), inner nuclear layer (INL) and inner plexiform layer (IPL). The red stain is Fox2, an RNA binding nuclear protein related to Fox3 / NeuN, which stains nuclei of horizontal neurons and some other neurons in the INL and IPL. Nuclear DNA was revealed with DAPI (blue).

  • ARG10696 anti-Arrestin 1 / S-Arrestin antibody [S128] WB image

    Western blot: Bovine retinal extracts stained with ARG10696 anti-Arrestin 1 / S-Arrestin antibody [S128].