ARG54152

anti-BRAP antibody

anti-BRAP antibody for Western blot and Human

Gene Regulation antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody recognizes BRAP
Tested Reactivity Hu
Tested Application WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name BRAP
Antigen Species Human
Immunogen Purified recombinant human BRAP protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names BRAP2; EC 6.3.2.-; RNF52; BRCA1-associated protein; Renal carcinoma antigen NY-REN-63; IMP; RING finger protein 52; Impedes mitogenic signal propagation

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 67 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1.8 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8315 Human BRAP

Swiss-port # Q7Z569 Human BRCA1-associated protein

Gene Symbol BRAP
Gene Full Name BRCA1 associated protein
Background Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.
Function Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport. [UniProt]
Cellular Localization Cytoplasm
Research Area Gene Regulation antibody; Signaling Transduction antibody
Calculated MW 67 kDa

Images (1) Click the Picture to Zoom In

  • ARG54152 anti-BRAP antibody WB image

    Western blot: A549 cell lysate stained with ARG54152 anti-BRAP antibody at 1:500 dilution.