ARG10775

anti-Bestrophin 3 antibody

anti-Bestrophin 3 antibody for Confocal microscopy (Confocal),ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot,Dot blot and Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Bestrophin 3
Tested Reactivity Ms, Rat
Tested Application Confocal, Dot, ELISA, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Bestrophin 3
Antigen Species Rat
Immunogen Synthetic peptide taken within the last 50 aa (C-terminus) from Rat Bestrophin 3.
Conjugation Un-conjugated
Full Name bestrophin 3
Alternate Names VMD2L3; Bestrophin-3; Vitelliform macular dystrophy 2-like protein 3

Application Instructions

Application Suggestion
Tested Application Dilution
Confocal1:200
Dot1:10000
ELISA1:10000
ICC/IF1:200
IHC-P1:200
IP1:200
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 76 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 382427 Mouse BEST3

Swiss-port # Q6H1V1 Mouse Bestrophin-3

Gene Symbol Best3
Gene Full Name bestrophin 3
Background BEST3 belongs to the bestrophin family of anion channels, which includes BEST1 (MIM 607854), the gene mutant in vitelliform macular dystrophy (VMD; MIM 153700), and 2 other BEST1-like genes, BEST2 (MIM 607335) and BEST4 (MIM 607336). Bestrophins are transmembrane (TM) proteins that share a homology region containing a high content of aromatic residues, including an invariant arg-phe-pro (RFP) motif. The bestrophin genes share a conserved gene structure, with almost identical sizes of the 8 RFP-TM domain-encoding exons and highly conserved exon-intron boundaries. Each of the 4 bestrophin genes has a unique 3-prime end of variable length (Stohr et al., 2002 [PubMed 12032738]; Tsunenari et al., 2003 [PubMed 12907679]).[supplied by OMIM, Mar 2008]
Function Forms calcium-sensitive chloride channels. Permeable to bicarbonate. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG10775 anti-Bestrophin 3 antibody IHC-P image

    Immunohistochemistry: PFA-fixed and paraffin-embedded Mouse glomerulus stained with ARG10775 anti-Bestrophin 3 antibody at 1:100 dilution. Incubation for 36 h at 4°C. Requires antigen retrieval.