ARG62717

anti-CD11b antibody [M1/70] (FITC)

anti-CD11b antibody [M1/70] (FITC) for Blocking,Flow cytometry,ICC/IF,IHC-Frozen sections and Mouse,Human,Rhesus Monkey,Baboon,Rabbit

MDSC Marker antibody; Myeloid-derived suppressor cell antibody

Overview

Product Description FITC-conjugated Rat Monoclonal antibody [M1/70] recognizes CD11b
Tested Reactivity Hu, Ms, Bb, R. Mk, Rb
Tested Application BL, FACS, ICC/IF, IHC-Fr
Host Rat
Clonality Monoclonal
Clone M1/70
Isotype IgG2b, kappa
Target Name CD11b
Antigen Species Mouse
Immunogen C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes
Conjugation FITC
Alternate Names MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
FACS< 1 µg/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16409 Mouse ITGAM

GeneID: 3684 Human ITGAM

Swiss-port # P11215 Human Integrin alpha-M

Background CD11b (integrin alpha M chain): Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Function CD11b: Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development. [UniProt]
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Research Area MDSC Marker antibody; Myeloid-derived suppressor cell antibody
Calculated MW 127 kDa

Images (2) Click the Picture to Zoom In

  • ARG62717 anti-CD11b antibody [M1/70] (FITC) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG62717 anti-CD11b antibody [M1/70] (FITC) and ARG22106 anti-Ly6G + Ly6C antibody [RB6-8C5] (PE).

  • ARG62717 anti-CD11b antibody [M1/70] (FITC) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG62717 anti-CD11b antibody [M1/70] (FITC) and ARG22106 anti-Ly6G + Ly6C antibody [RB6-8C5] (PE).

Clone References

Experimental autoimmune prostatitis induces microglial activation in the spinal cord.

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Tetrandrine suppresses lipopolysaccharide-induced microglial activation by inhibiting NF-κB and ERK signaling pathways in BV2 cells.

WB / Mouse

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PLoS One.,  (2014)

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VEGFR-1 blockade disrupts peri-implantation decidual angiogenesis and macrophage recruitment.

IHC / Mouse

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Astrocyte-derived BDNF supports myelin protein synthesis after cuprizone-induced demyelination.

IHC-FoFr / Mouse

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Role of cysteine-rich 61 protein (CCN1) in macrophage-mediated oncolytic herpes simplex virus clearance.

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Retinoid-X-receptors (α/β) in melanocytes modulate innate immune responses and differentially regulate cell survival following UV irradiation.

ICC/IF / Mouse

Coleman DJ et al.
PLoS Genet.,  (2014)

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Gr-1+CD11b+ cells facilitate Lewis lung cancer recurrence by enhancing neovasculature after local irradiation.

IHC-Fr / Mouse

Liu T et al.
Sci Rep.,  (2014)

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Intrastriatal transplantation of adenovirus-generated induced pluripotent stem cells for treating neuropathological and functional deficits in a rodent model of Huntington's disease.

Fink KD et al.
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Neurons are host cells for Mycobacterium tuberculosis.

Randall PJ et al.
Infect Immun.,  (2014)

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MCP-1/CCR2 signaling-mediated astrocytosis is accelerated in a transgenic mouse model of SOD1-mutated familial ALS.

IHC-FoFr / Mouse

Kawaguchi-Niida M et al.
Acta Neuropathol Commun.,  (2013)

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Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia.

FACS / Mouse

Olme CH et al.
Leuk Res.,  (2013)

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Intravenous immunglobulin binds beta amyloid and modifies its aggregation, neurotoxicity and microglial phagocytosis in vitro.

ICC/IF / Mouse

Cattepoel S et al.
PLoS One.,  (2013)

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Junctional adhesion molecule-A regulates vascular endothelial growth factor receptor-2 signaling-dependent mouse corneal wound healing.

IHC-Fr / Mouse

Chatterjee S et al.
PLoS One.,  (2013)

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Matricellular protein CCN1 activates a proinflammatory genetic program in murine macrophages.

FuncSt / Mouse

Bai T et al.
J Immunol.,  (2010)

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Surfactant protein A modulates cell surface expression of CR3 on alveolar macrophages and enhances CR3-mediated phagocytosis.

FACS / 

Gil M et al.
J Biol Chem.,  (2009)

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Particulate matter exposure induces persistent lung inflammation and endothelial dysfunction.

IHC-Fr / Rabbit

Tamagawa E et al.
Am J Physiol Lung Cell Mol Physiol.,  (2008)

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