anti-CD11b antibody [MEM-170] (Functional Grade)

anti-CD11b antibody [MEM-170] (Functional Grade) for Flow cytometry,Functional study and Human

MDSC Marker antibody; Myeloid-derived suppressor cell antibody


Product Description

Functional Grade Mouse Monoclonal antibody [MEM-170] recognizes CD11b

Tested Reactivity Hu
Tested Application FACS, FuncSt
Specificity The clone MEM-170 recognizes CD11b antigen (Mac-1alpha), a 165-170 kDa transmembrane protein type 1 mainly expressed on monocytes, granulocytes and NK-cells. blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen.
HLDA VI., WS Code A054
Host Mouse
Clonality Monoclonal
Clone MEM-170
Isotype IgG1
Target Name CD11b
Antigen Species Human
Immunogen Human granulocytes
Conjugation Un-conjugated
Alternate Names MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 µg/ml
Application Note Functional studies: The clone MEM-170 blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 3684 Human ITGAM

Swiss-port # P11215 Human Integrin alpha-M

Gene Symbol ITGAM
Gene Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Background CD11b (integrin alpha M chain): Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Function CD11b: Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development. [UniProt]
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Research Area MDSC Marker antibody; Myeloid-derived suppressor cell antibody
Calculated MW 127 kDa

Images (1) Click the Picture to Zoom In

  • ARG62713 anti-CD11b antibody [MEM-170] (Functional Grade) FACS image

    Flow Cytometry: Human peripheral blood cells stained with ARG62713 anti-CD11b antibody [MEM-170] (Functional Grade), followed by incubation with APC labelled Goat anti-Mouse secondary antibody.

Clone References

A systemic lupus erythematosus-associated R77H substitution in the CD11b chain of the Mac-1 integrin compromises leukocyte adhesion and phagocytosis.

WB / Human

MacPherson M et al.
J Biol Chem.,  (2011)




alpha-Chain phosphorylation of the human leukocyte CD11b/CD18 (Mac-1) integrin is pivotal for integrin activation to bind ICAMs and leukocyte extravasation.

Fagerholm SC et al.
Blood.,  (2006)