anti-CD13 antibody [WM15] (PerCP)

anti-CD13 antibody [WM15] (PerCP) for Flow cytometry and Human,Primates

Developmental Biology antibody; Immune System antibody


Product Description

PerCP-conjugated Mouse Monoclonal antibody [WM15] recognizes CD13

Tested Reactivity Hu, NHuPrm
Tested Application FACS
Specificity The clone WM15 recognises the human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors.
HLDA III; WS Code M 213
HLDA IV; WS Code M 44
HLDA IV; WS Code M 209
HLDA V; WS Code M MA191
Host Mouse
Clonality Monoclonal
Clone WM15
Isotype IgG1
Target Name CD13
Antigen Species Human
Immunogen Human AML cells
Conjugation PerCP
Alternate Names AP-N; PEPN; LAP1; CD antigen CD13; Aminopeptidase M; gp150; Aminopeptidase N; EC; Myeloid plasma membrane glycoprotein CD13; APN; CD13; P150; AP-M; GP150; hAPN; Microsomal aminopeptidase; Alanyl aminopeptidase

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 290 Human ANPEP

Swiss-port # P15144 Human Aminopeptidase N

Gene Symbol ANPEP
Gene Full Name alanyl (membrane) aminopeptidase
Background CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.
Function Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 110 kDa
PTM Sulfated.
N- and O-glycosylated.
May undergo proteolysis and give rise to a soluble form.

Images (1) Click the Picture to Zoom In

  • ARG54312 anti-CD13 antibody [WM15] (PerCP) FACS image

    Flow Cytometry: Human peripheral blood leukocytes stained with ARG54312 anti-CD13 antibody [WM15] (PerCP).

Clone References

Identification of prostate-enriched proteins by in-depth proteomic analyses of expressed prostatic secretions in urine.

WB / 

Principe S et al.
J Proteome Res.,  (2012)




The hepatobiliary disease marker serum alanine aminopeptidase predominantly comprises an isoform of the haematological myeloid differentiation antigen and leukaemia marker CD-13/gp150.

Favaloro EJ et al.
Clin Chim Acta.,  (1993)




CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated.

Favaloro EJ et al.
Exp Hematol.,  (1993)




CD-13 ('gp150'; aminopeptidase-N): co-expression on endothelial and haemopoietic cells with conservation of functional activity.

Favaloro EJ et al.
Immunol Cell Biol.,  (1991)