anti-CD20 antibody [2H7] (PerCP)

anti-CD20 antibody [2H7] (PerCP) for Flow cytometry and Human,Primates

Cancer antibody; Developmental Biology antibody; Immune System antibody; B cell Marker antibody; Immature B Cell Marker antibody; Inflammatory Cell Marker antibody; Tumor-infiltrating Lymphocyte Study antibody


Product Description PerCP-conjugated Mouse Monoclonal antibody [2H7] recognizes CD20
Tested Reactivity Hu, NHuPrm
Tested Application FACS
Specificity The mouse monoclonal antibody 2H7 recognizes CD20 (B1, Bp35), a 3337 kDa nonglycosylated membrane receptor with four transmembrane domains, expressed on preB lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.
Host Mouse
Clonality Monoclonal
Clone 2H7
Isotype IgG2b
Target Name CD20
Antigen Species Human
Immunogen Human tonsillar B cells
Conjugation PerCP
Alternate Names Bp35; LEU-16; B-lymphocyte surface antigen B1; B-lymphocyte antigen CD20; CD20; S7; CD antigen CD20; Leukocyte surface antigen Leu-16; B1; CVID5; Membrane-spanning 4-domains subfamily A member 1; MS4A2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 931 Human MS4A1

Swiss-port # P11836 Human B-lymphocyte antigen CD20

Gene Symbol MS4A1
Gene Full Name membrane-spanning 4-domains, subfamily A, member 1
Background CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]
Function CD20 is a B-lymphocyte-specific membrane protein. It plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602). [UniProt]
Highlight Related products:
CD20 antibodies; CD20 ELISA Kits; CD20 Duos / Panels; Anti-Mouse IgG secondary antibodies;
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Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody; B cell Marker antibody; Immature B Cell Marker antibody; Inflammatory Cell Marker antibody; Tumor-infiltrating Lymphocyte Study antibody
Calculated MW 33 kDa
PTM Phosphorylated. Might be functionally regulated by protein kinase(s).

Images (1) Click the Picture to Zoom In

  • ARG54199 anti-CD20 antibody [2H7] (PerCP) FACS image

    Flow Cytometry: Human peripheral blood stained with ARG54199 anti-CD20 antibody [2H7] (PerCP).

Clone References

CD20-directed small modular immunopharmaceutical, TRU-015, depletes normal and malignant B cells.

Hayden-Ledbetter MS et al.
Clin Cancer Res.,  (2009)




Novel follicular dendritic cell molecule, 8D6, collaborates with CD44 in supporting lymphomagenesis by a Burkitt lymphoma cell line, L3055.

Li L et al.
Blood.,  (2004)




Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure.

Polyak MJ et al.
Blood.,  (2002)




Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment.

Deans JP et al.
J Biol Chem.,  (1998)




Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20.

Deans JP et al.
J Biol Chem.,  (1995)