ARG21279

anti-CD20 antibody [B-Ly1] (PE-Cyanine 7)

anti-CD20 antibody [B-Ly1] (PE-Cyanine 7) for Blocking,Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Human

Cancer antibody; Developmental Biology antibody; Immune System antibody; B cell Marker antibody; Immature B Cell Marker antibody; Inflammatory Cell Marker antibody; Tumor-infiltrating Lymphocyte Study antibody

Overview

Product Description PE-Cyanine 7-conjugated Mouse Monoclonal antibody [B-Ly1] recognizes CD20
Tested Reactivity Hu
Tested Application BL, FACS, IHC-Fr, IHC-P
Specificity Human CD20
Host Mouse
Clonality Monoclonal
Clone B-Ly1
Isotype IgG1, kappa
Target Name CD20
Antigen Species Human
Conjugation PE-Cyanine 7
Full Name membrane-spanning 4-domains, subfamily A, member 1
Alternate Names Bp35; LEU-16; B-lymphocyte surface antigen B1; B-lymphocyte antigen CD20; CD20; S7; CD antigen CD20; Leukocyte surface antigen Leu-16; B1; CVID5; Membrane-spanning 4-domains subfamily A member 1; MS4A2

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
FACS10 µl/10^6 cells
IHC-FrAssay-dependent
IHC-PAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 33 kDa

Properties

Form Liquid
Buffer PBS, Sodium azide and a stabilizing agent
Preservative Sodium azide
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 931 Human MS4A1

Swiss-port # P11836 Human B-lymphocyte antigen CD20

Gene Symbol MS4A1
Gene Full Name membrane-spanning 4-domains, subfamily A, member 1
Background CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]
Function CD20 is a B-lymphocyte-specific membrane protein. It plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602). [UniProt]
Highlight Related products:
CD20 antibodies; CD20 ELISA Kits; CD20 Duos / Panels; Anti-Mouse IgG secondary antibodies;
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Tumor-Infiltrating Lymphocytes (TILs)
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Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody; B cell Marker antibody; Immature B Cell Marker antibody; Inflammatory Cell Marker antibody; Tumor-infiltrating Lymphocyte Study antibody
PTM Phosphorylated. Might be functionally regulated by protein kinase(s).

Images (1) Click the Picture to Zoom In

  • ARG21279 anti-CD20 antibody [B-Ly1] (PE-Cyanine 7) FACS image

    Flow Cytometry: Human peripheral blood lymphocytes stained with ARG21279 anti-CD20 antibody [B-Ly1] (PE-Cyanine 7) and ARG21260 anti-CD19 antibody [SJ25-C1] (PE).