ARG56003

anti-CD20 antibody [L26]

anti-CD20 antibody [L26] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Baboon,Human,Monkey

Cancer antibody; Developmental Biology antibody; Immune System antibody; B cell Marker antibody; Immature B Cell Marker antibody; Inflammatory Cell Marker antibody; Tumor-infiltrating Lymphocyte Study antibody

Overview

Product Description

Mouse Monoclonal antibody [L26] recognizes CD20

Tested Reactivity Hu, Bb, Mk
Species Does Not React With Rat, Cow, Dog, Pig
Tested Application FACS, ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone L26
Isotype IgG2a, kappa
Target Name CD20
Antigen Species Human
Immunogen Human tonsil B cells.
Conjugation Un-conjugated
Alternate Names Bp35; LEU-16; B-lymphocyte surface antigen B1; B-lymphocyte antigen CD20; CD20; S7; CD antigen CD20; Leukocyte surface antigen Leu-16; B1; CVID5; Membrane-spanning 4-domains subfamily A member 1; MS4A2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS5 - 10 µl/10^6 cells
ICC/IF1:50 - 1:100
IHC-P1:100 - 1:200
WB1:50 - 1:100
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM Citrate buffer (pH 6.0) for 10-20 min followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS (pH 7.4) and 0.05% Sodium azide
Preservative 0.05% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 931 Human MS4A1

Swiss-port # P11836 Human B-lymphocyte antigen CD20

Gene Symbol MS4A1
Gene Full Name membrane-spanning 4-domains, subfamily A, member 1
Background CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]
Function CD20 is a B-lymphocyte-specific membrane protein. It plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602). [UniProt]
Cellular Localization Predominantly cell surface with some cytoplasmic
Highlight Related products:
CD20 antibodies; CD20 ELISA Kits; CD20 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Tumor-Infiltrating Lymphocytes (TILs)
New antibody panels and duos for Tumor immune microenvironment
Exploring Antiviral Immune Response
Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody; B cell Marker antibody; Immature B Cell Marker antibody; Inflammatory Cell Marker antibody; Tumor-infiltrating Lymphocyte Study antibody
Calculated MW 33 kDa
PTM Phosphorylated. Might be functionally regulated by protein kinase(s).

Images (3) Click the Picture to Zoom In

  • ARG56003 anti-CD20 antibody [L26] IHC-P image

    Immunohistochemistry: Formalin-fixed, paraffin-embedded Human tonsil tissue stained with ARG56003 anti-CD20 antibody [L26].

  • ARG56003 anti-CD20 antibody [L26] WB image

    Western blot: Ramos cell lysate stained with ARG56003 anti-CD20 antibody [L26].

  • ARG56003 anti-CD20 antibody [L26] IHC-P image

    Immunohistochemistry: Human lymphoma tissue stained with ARG56003 anti-CD20 antibody [L26].