ARG20873

anti-CD23 antibody [2G8]

anti-CD23 antibody [2G8] for ELISA,Flow cytometry,IHC-Frozen sections and Mouse

Overview

Product Description Rat Monoclonal antibody [2G8] recognizes CD23
Tested Reactivity Ms
Tested Application ELISA, FACS, IHC-Fr
Specificity Mouse CD23.
Host Rat
Clonality Monoclonal
Clone 2G8
Isotype IgG2a, kappa
Target Name CD23
Species Mouse
Immunogen FcεR isolated from B cell lymphoma cell line M12.4.5
Conjugation Un-conjugated
Full Name Fc receptor, IgE, low affinity II, alpha polypeptide
Alternate Names CD23A; BLAST-2; Lymphocyte IgE receptor; CLEC4J; Immunoglobulin E-binding factor; CD23; IGEBF; Fc-epsilon-RII; CD antigen CD23; FCE2; Low affinity immunoglobulin epsilon Fc receptor; C-type lectin domain family 4 member J

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS< 1 µg/10^6 cells
IHC-FrAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 36 kDa

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol FCER2
Gene Full Name Fc receptor, IgE, low affinity II, alpha polypeptide
Background The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
Function Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). [UniProt]
PTM N- and O-glycosylated.
The secreted form sCD23 is produced by ADAM10-mediated ectodomain shedding.

Images (1) Click the Picture to Zoom In

  • ARG20873 anti-CD23 antibody [2G8] FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG20873 anti-CD23 antibody [2G8] and ARG20819 anti-CD3e antibody [C363.29B] (PE) followed by ARG21700 Mouse anti-Rat IgG2a antibody [2A8F4] (FITC).