ARG20892

anti-CD25 antibody [3C7] (PE-Cyanine 5)

anti-CD25 antibody [3C7] (PE-Cyanine 5) for Blocking,Flow cytometry,IHC-Frozen sections,Neutralizing and Mouse

Immune System antibody; Pre-B Cell Marker antibody

Overview

Product Description PE-Cyanine 5-conjugated Rat Monoclonal antibody [3C7] recognizes CD25
Tested Reactivity Ms
Tested Application BL, FACS, IHC-Fr, Neut
Specificity Mouse CD25. The clone 3C7 reacts with an epitope of CD25 which is distinct from that recognized by clone 7D4. 3C7, used in combination with 7D4 in culture, results in higher levels of inhibition of proliferation driven by IL-2 and generation of alloreactive CTL than either of these monoclonal antibodies alone.
Host Rat
Clonality Monoclonal
Clone 3C7
Isotype IgG2b, kappa
Target Name CD25
Species Mouse
Immunogen IL-2 dependent BALB/c helper T cell clone HT2
Conjugation PE-Cyanine 5
Full Name interleukin 2 receptor, alpha chain
Alternate Names IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
FACS< 0.3 µg/10^6 cells
IHC-FrAssay-dependent
NeutAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 31 kDa

Properties

Form Liquid
Buffer PBS, Sodium azide and a stabilizing agent
Preservative Sodium azide
Concentration 0.1 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 16184 Mouse IL2RA

Swiss-port # P01590 Mouse Interleukin-2 receptor subunit alpha

Gene Symbol IL2RA
Gene Full Name interleukin 2 receptor, alpha chain
Background The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
Function Receptor for interleukin-2. [UniProt]
Research Area Immune System antibody; Pre-B Cell Marker antibody

Images (1) Click the Picture to Zoom In

  • ARG20892 anti-CD25 antibody [3C7] (PE-Cyanine 5) FACS image

    Flow Cytometry: C57BL/6 Mouse cytotoxic T lymphocyte cell line CTLL-2 was stained with ARG20892 anti-CD25 antibody [3C7] (PE-Cyanine 5).