anti-CD33 antibody [WM53] (PE-Cyanine 5)

anti-CD33 antibody [WM53] (PE-Cyanine 5) for Flow cytometry,IHC-Frozen sections,ICC/IF,Western blot,Blocking and Human

Developmental Biology antibody; Immune System antibody; Human MDSC Marker antibody; Myeloid-derived suppressor cell antibody


Product Description PE-Cyanine 5-conjugated Mouse Monoclonal antibody [WM53] recognizes CD33
Tested Reactivity Hu
Tested Application BL, FACS, ICC/IF, IHC-Fr, WB
Specificity Human CD33
Host Mouse
Clonality Monoclonal
Clone WM53
Isotype IgG1, kappa
Target Name CD33
Antigen Species Human
Immunogen Human AML cells.
Conjugation PE-Cyanine 5
Alternate Names p67; Sialic acid-binding Ig-like lectin 3; SIGLEC-3; CD antigen CD33; gp67; Siglec-3; Myeloid cell surface antigen CD33; SIGLEC3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl/10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Buffer PBS, 0.1% Sodium azide and Sucrose.
Preservative 0.1% Sodium azide
Stabilizer Sucrose
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 945 Human CD33

Swiss-port # P20138 Human Myeloid cell surface antigen CD33

Gene Symbol CD33
Gene Full Name CD33 molecule
Background CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.
Function CD33: Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:15597323, PubMed:11320212). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed:7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:28325905, PubMed:10887109). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10556798, PubMed:10206955, PubMed:10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323). [UniProt]
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Research Area Developmental Biology antibody; Immune System antibody; Human MDSC Marker antibody; Myeloid-derived suppressor cell antibody
Calculated MW 40 kDa
PTM Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.