anti-CD44 antibody [MEM-85] (PE)

anti-CD44 antibody [MEM-85] (PE) for Flow cytometry and Human

Cancer antibody; Developmental Biology antibody; Immune System antibody; Chondrogenesis Study antibody


Product Description PE-conjugated Mouse Monoclonal antibody [MEM-85] recognizes CD44
Tested Reactivity Hu
Tested Application FACS
Specificity The clone MEM-85 reacts with both cell surface-expressed and soluble form of CD44 antigen (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.
HLDA IV; WS Code NL 706
HLDA VI; WS Code AS Ref.15
Host Mouse
Clonality Monoclonal
Clone MEM-85
Isotype IgG2b
Target Name CD44
Immunogen Leukocytes of a patient suffering from LGL Type Leukaemia.
Conjugation PE
Alternate Names MDU2; MDU3; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; Extracellular matrix receptor III; PGP-I; Epican; CDW44; Phagocytic glycoprotein 1; Pgp1; HUTCH-I; MC56; Hyaluronate receptor; CD antigen CD44; Heparan sulfate proteoglycan; CD44 antigen; LHR; IN; HCELL; Phagocytic glycoprotein I; PGP-1; CSPG8; MIC4; ECMR-III; CDw44

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 960 Human CD44

Swiss-port # P16070 Human CD44 antigen

Gene Symbol CD44
Gene Full Name CD44 molecule (Indian blood group)
Background CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.
Function Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. [UniProt]
Highlight Related products:
CD44 antibodies; CD44 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Stem cell and the regenerative medicine: Ready for the patients
Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody; Chondrogenesis Study antibody
Calculated MW 82 kDa
PTM Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N- and O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638.
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.

Images (1) Click the Picture to Zoom In

  • ARG53852 anti-CD44 antibody [MEM-85] (PE) FACS image

    Flow Cytometry: Human peripheral blood cells stained with ARG53852 anti-CD44 antibody [MEM-85] (PE).

Clone References

Umbilical cord mesenchymal stem cells suppress host rejection: the role of the glycocalyx.

ICC/IF / Human

Coulson-Thomas VJ et al.
J Biol Chem.,  (2014)




Organization and regulation of intracellular plasma membrane-connected HIV-1 assembly compartments in macrophages.

ICC/IF / Human

Mlcochova P et al.
BMC Biol.,  (2013)




Human leucocyte surface glycoprotein CDw44 and lymphocyte homing receptor are identical molecules.

Stefanová I et al.
Immunogenetics.,  (1989)




Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.

Bazil V et al.
Folia Biol (Praha).,  (1989)