anti-CD58 antibody [MEM-63] (PE)

anti-CD58 antibody [MEM-63] (PE) for Flow cytometry and Human,Pig

Immune System antibody


Product Description PE-conjugated Mouse Monoclonal antibody [MEM-63] recognizes CD58
Tested Reactivity Hu, Pig
Tested Application FACS
Specificity The clone MEM-63 reacts with CD58 (LFA-3), a 40-70 kDa glycoprotein distributed over many tissues, leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts.
HLDA VI; WS Code AS A047
Host Mouse
Clonality Monoclonal
Clone MEM-63
Isotype IgG1
Target Name CD58
Antigen Species Human
Immunogen NALM-6 human pre-B cell line
Conjugation PE
Alternate Names CD antigen CD58; LFA3; ag3; Lymphocyte function-associated antigen 3; Surface glycoprotein LFA-3; Ag3; LFA-3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer TBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 965 Human CD58

Swiss-port # P19256 Human Lymphocyte function-associated antigen 3

Gene Symbol CD58
Gene Full Name CD58 molecule
Background CD58 (LFA-3) is an immunoglobulin family adhession molecule expressed by both hematopoietic and non-hematopoietic cells (often on antigen presenting cells) and serving as ligand of CD2. This interaction is important for T cell-mediated immunity. CD58 is expressed in transmembrane form and in GPI-anchored form; the later is constitutively associated with protein kinases whereas the transmembrane form activates kinase activity upon triggering. CD58 is a powerful tool for detection of minimal residual disease in acute lymphocytic leukemia, and for evaluation of liver damage related with hepatitis B.
Function Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. [UniProt]
Research Area Immune System antibody
Calculated MW 28 kDa

Images (1) Click the Picture to Zoom In

  • ARG53880 anti-CD58 antibody [MEM-63] (PE) FACS image

    Flow Cytometry: Human peripheral blood cells stained with ARG53880 anti-CD58 antibody [MEM-63] (PE).