ARG53887

anti-CD63 antibody [MEM-259] (PE)

anti-CD63 antibody [MEM-259] (PE) for Flow cytometry and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Immune System antibody

Overview

Product Description

PE-conjugated Mouse Monoclonal antibody [MEM-259] recognizes CD63

Tested Reactivity Hu
Tested Application FACS
Specificity The clone MEM-259 reacts with CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.
Host Mouse
Clonality Monoclonal
Clone MEM-259
Isotype IgG1
Target Name CD63
Immunogen HPB-ALL T cell line
Conjugation PE
Full Name CD63 molecule
Alternate Names Tspan-30; CD63 antigen; Tetraspanin-30; CD antigen CD63; Lysosomal-associated membrane protein 3; OMA81H; Ocular melanoma-associated antigen; Granulophysin; TSPAN30; Melanoma-associated antigen ME491; MLA1; LAMP-3; ME491

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 967 Human CD63

Swiss-port # P08962 Human CD63 antigen

Gene Symbol CD63
Gene Full Name CD63 molecule
Background CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.
Function Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli. [UniProt]
Highlight Related products:
CD63 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
Tools for studying Exosomes
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Immune System antibody
PTM Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro).

Images (1) Click the Picture to Zoom In

  • ARG53887 anti-CD63 antibody [MEM-259] (PE) FACS image

    Flow Cytometry: IgE-activated peripheral blood stained with ARG53887 anti-CD63 antibody [MEM-259] (PE).

Clone References

The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling.

IHC / Human

Van Deun J et al.
J Extracell Vesicles.,  (2014)

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Exosomes from hepatitis C infected patients transmit HCV infection and contain replication competent viral RNA in complex with Ago2-miR122-HSP90.

Bukong TN et al.
PLoS Pathog.,  (2014)

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Investigation of soluble and transmembrane CTLA-4 isoforms in serum and microvesicles.

EM / Human

Esposito L et al.
J Immunol.,  (2014)

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A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration.

WB / Human

Salomon C et al.
PLoS One.,  (2014)

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Epstein-Barr virus-encoded small RNAs (EBERs) are present in fractions related to exosomes released by EBV-transformed cells.

WB / Human

Ahmed W et al.
PLoS One.,  (2014)

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A human blood-brain barrier transcytosis assay reveals antibody transcytosis influenced by pH-dependent receptor binding.

ICC/IF / Human

Sade H et al.
PLoS One.,  (2014)

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Syntenin-ALIX exosome biogenesis and budding into multivesicular bodies are controlled by ARF6 and PLD2.

Ghossoub R et al.
Nat Commun.,  (2014)

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