anti-CD63 antibody [NKI/C3]

anti-CD63 antibody [NKI/C3] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse

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Product Description

Mouse Monoclonal antibody [NKI/C3] recognizes CD63

Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF, IHC-P
Host Mouse
Clonality Monoclonal
Clone NKI/C3
Isotype IgG1, kappa
Target Name CD63
Antigen Species Human
Immunogen Smooth plasma membrane fraction of MeWo cells.
Conjugation Un-conjugated
Alternate Names Tspan-30; CD63 antigen; Tetraspanin-30; CD antigen CD63; Lysosomal-associated membrane protein 3; OMA81H; Ocular melanoma-associated antigen; Granulophysin; TSPAN30; Melanoma-associated antigen ME491; MLA1; LAMP-3; ME491

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 2 µg/10^6 cells
ICC/IF1 - 2 µg/ml
IHC-P1 - 2 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 12512 Mouse CD63

GeneID: 967 Human CD63

Swiss-port # P08962 Human CD63 antigen

Swiss-port # P41731 Mouse CD63 antigen

Gene Symbol CD63
Gene Full Name CD63 molecule
Background The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]
Function Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli. [UniProt]
Cellular Localization Cytoplasmic
Highlight Related products:
CD63 antibodies; Anti-Mouse IgG secondary antibodies;
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Calculated MW 26 kDa
PTM Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro).

Images (4) Click the Picture to Zoom In

  • ARG55941 anti-CD63 antibody [NKI/C3] ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG55941 anti-CD63 antibody [NKI/C3] at 1:200 dilution. Left: primary antibody (green). Right: primary antibody and DAPI (Merge).

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG55941 anti-CD63 antibody [NKI/C3] FACS image

    Flow Cytometry: MCF-7 cells stained with PE-conjugated ARG55941 anti-CD63 antibody [NKI/C3] (red); Cells alone (black); Isotype control (green).

  • ARG55941 anti-CD63 antibody [NKI/C3] IHC-P image

    Immunohistochemistry: Human melanoma stained with ARG55941 anti-CD63 antibody [NKI/C3].

  • ARG55941 anti-CD63 antibody [NKI/C3] IHC-P image

    Immunohistochemistry: Mouse spleen stained with ARG55941 anti-CD63 antibody [NKI/C3].

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Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC

Specific References

miRNA-181a over-expression in mesenchymal stem cell-derived exosomes influenced inflammatory response after myocardial ischemia-reperfusion injury.

WB / Human

Wei Zilun et al.
Life Sci.,  (2019)