ARG10514

anti-CD68 antibody [KP1]

anti-CD68 antibody [KP1] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunohistochemistry (PFA perfusion fixed frozen sections),Western blot and Human,Mouse,Rabbit,Rat

Immune System antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Active macroglial Marker antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody; Inflammatory Cell Marker antibody
publication_link Publication2

Overview

Product Description

Mouse Monoclonal antibody [KP1] recognizes CD68

Tested Reactivity Hu, Ms, Rat, Rb
Tested Application FACS, ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone KP1
Isotype IgG1
Target Name CD68
Immunogen Lysosomal contents of lung macrophages.
Conjugation Un-conjugated
Full Name CD68 molecule
Alternate Names Macrosialin; CD antigen CD68; LAMP4; Gp110; GP110; SCARD1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:100 - 1:1000 for 10^6 cells in 100 μl
ICC/IFAssay-dependent
IHC-FoFr Assay-dependent
IHC-Fr1:200 - 1:400.
IHC-P1:200 - 1:400
WBAssay-dependent
Application Note IHC-P: Antigen Retrieval: Boil tissue section in Tris-EDTA pH9.0 or Citrate buffer (pH 6.0). Incubate with antibody at 4°C for overnight.
IHC-Fr: Incubate with the antibody at 4°C for overnight or at RT for 30-60 mins.
Flow cytometry: Membrane permeabilisation is required for this application.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 37 kDa
Observed Size ~ 110 kDa

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 12514 Mouse CD68

GeneID: 968 Human CD68

Swiss-port # P31996 Mouse Macrosialin

Swiss-port # P34810 Human Macrosialin

Gene Symbol CD68
Gene Full Name CD68 molecule
Background CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
Function CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30317 Activated Macrophage / Microglia Marker Antibody Duo
ARG30324 Neuroinflammation Antibody Panel
ARG30333 M1/M2/TAM Marker Antibody Panel
Related products:
CD68 antibodies; CD68 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
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Tumor-Infiltrating Lymphocytes (TILs)
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Research Area Immune System antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Active macroglial Marker antibody; M1/M2/TAM Marker antibody; Macrophage Marker antibody; M1 macrophage Marker antibody; Inflammatory Cell Marker antibody
PTM N- and O-glycosylated.

Images (5) Click the Picture to Zoom In

  • ARG10514 anti-CD68 antibody [KP1] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil stained with ARG10514 anti-CD68 antibody [KP1]. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).

  • ARG10514 anti-CD68 antibody [KP1] WB image

    Western blot: Human Macrophages and N1 Fibroblast untreated or treated with N-glycosidase F. The blots were stained with ARG10514 anti-CD68 antibody [KP1].

  • ARG10514 anti-CD68 antibody [KP1] FACS image

    Flow Cytometry: THP1 cells prefixed with 4% PFA and then permeabilised with 0.25% saponin. Cells were stained with ARG10514 anti-CD68 antibody [KP1] (white area) or isotype control antibody (gray area).

  • ARG10514 anti-CD68 antibody [KP1] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast carcinoma stained with ARG10514 anti-CD68 antibody [KP1].

  • ARG10514 anti-CD68 antibody [KP1] IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse kidney stained with ARG10514 anti-CD68 antibody [KP1] at 1:200 dilution.

    Secondary antibody: Anti-Mouse IgG antibody (Rhodamine red).

Specific References

TAB1 regulates glycolysis and activation of macrophages in diabetic nephropathy.

IHC-P / Mouse

Hanxu Zeng et al.
Inflamm Res.,  (2020)

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Exosomes from high glucose-treated macrophages activate glomerular mesangial cells via TGF-β1/Smad3 pathway in vivo and in vitro.

IHC-P / Mouse

Zhu Qi-jin et al.
FASEB J.,  (2019)

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