ARG10514

anti-CD68 antibody [KP1]

anti-CD68 antibody [KP1] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunohistochemistry (PFA perfusion fixed frozen sections),Western blot and Human,Mouse,Rabbit,Rat

Immune System antibody
publication_link Publication1

Overview

Product Description

Mouse Monoclonal antibody [KP1] recognizes CD68

Tested Reactivity Hu, Ms, Rat, Rb
Tested Application FACS, ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone KP1
Isotype IgG1
Target Name CD68
Immunogen Lysosomal contents of lung macrophages.
Conjugation Un-conjugated
Full Name CD68 molecule
Alternate Names Macrosialin; CD antigen CD68; LAMP4; Gp110; GP110; SCARD1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:100 - 1:1000 for 10^6 cells in 100 μl
ICC/IFAssay-dependent
IHC-FoFr Assay-dependent
IHC-Fr1:200 - 1:400. (30-60 mins, RT)
IHC-P1:200 - 1:400
WBAssay-dependent
Application Note IHC-P: Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 110 kDa

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 12514 Mouse CD68

GeneID: 968 Human CD68

Swiss-port # P31996 Mouse Macrosialin

Swiss-port # P34810 Human Macrosialin

Gene Symbol CD68
Gene Full Name CD68 molecule
Background CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. KP1 is widely used to detect macrophages in paraffin embedded tissue sections.
Function Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. [Uniprot]
Highlight Related products:
CD68 antibodies; CD68 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Tumor-Infiltrating Lymphocytes (TILs)
Research Area Immune System antibody
PTM N- and O-glycosylated.

Images (5) Click the Picture to Zoom In

  • ARG10514 anti-CD68 antibody [KP1] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil stained with ARG10514 anti-CD68 antibody [KP1]. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).

  • ARG10514 anti-CD68 antibody [KP1] WB image

    Western blot: Human Macrophages and N1 Fibroblast untreated or treated with N-glycosidase F. The blots were stained with ARG10514 anti-CD68 antibody [KP1].

  • ARG10514 anti-CD68 antibody [KP1] FACS image

    Flow Cytometry: THP1 cells prefixed with 4% PFA and then permeabilised with 0.25% saponin. Cells were stained with ARG10514 anti-CD68 antibody [KP1] (white area) or isotype control antibody (gray area).

  • ARG10514 anti-CD68 antibody [KP1] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast carcinoma stained with ARG10514 anti-CD68 antibody [KP1].

  • ARG10514 anti-CD68 antibody [KP1] IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse kidney stained with ARG10514 anti-CD68 antibody [KP1] at 1:200 dilution.

    Secondary antibody: Anti-Mouse IgG antibody (Rhodamine red).

Specific References

Exosomes from high glucose-treated macrophages activate glomerular mesangial cells via TGF-β1/Smad3 pathway in vivo and in vitro.

WB / Mouse

Zhu Qi-jin et al.
FASEB J.,  (2019)

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