anti-CD8 antibody [RFT8] (PE/Texas Red)

anti-CD8 antibody [RFT8] (PE/Texas Red) for Depletion,Flow cytometry,ICC/IF,IHC-Frozen sections and Human

Developmental Biology antibody; Immune System antibody; Cytotoxic T antibody; Cytotoxic T Cell Surface Study antibody; Tumor-infiltrating Lymphocyte Study antibody


Product Description PE/Texas Red-conjugated Mouse Monoclonal antibody [RFT8] recognizes CD8
Tested Reactivity Hu
Tested Application Depletion, FACS, ICC/IF, IHC-Fr
Specificity Human CD8.
Host Mouse
Clonality Monoclonal
Clone RFT8
Isotype IgG1, kappa
Target Name CD8
Antigen Species Human
Immunogen Thymocytes and E-rosetted lymphocytes
Conjugation PE/Texas Red
Alternate Names T-cell surface glycoprotein CD8 alpha chain; Leu2; p32; T-lymphocyte differentiation antigen T8/Leu-2; CD8; MAL; CD antigen CD8a

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl/10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Buffer PBS, 0.1% Sodium azide and Sucrose.
Preservative 0.1% Sodium azide
Stabilizer Sucrose
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 925 Human CD8A

Swiss-port # P01732 Human T-cell surface glycoprotein CD8 alpha chain

Gene Symbol CD8A
Gene Full Name CD8a molecule
Background CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Function CD8 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. [UniProt]
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Research Area Developmental Biology antibody; Immune System antibody; Cytotoxic T antibody; Cytotoxic T Cell Surface Study antibody; Tumor-infiltrating Lymphocyte Study antibody
Calculated MW 26 kDa
PTM All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.

Images (1) Click the Picture to Zoom In

  • ARG21314 anti-CD8 antibody [RFT8] (PE/Texas Red) FACS image

    Flow Cytometry: Human peripheral blood lymphocytes stained with ARG21314 anti-CD8 antibody [RFT8] (PE/Texas Red) and ARG21298 anti-CD4 antibody [RFT4] (PE).