anti-CD82 antibody [C33]

anti-CD82 antibody [C33] for Flow cytometry,Functional study,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Cancer antibody; Gene Regulation antibody; Immune System antibody


Product Description Mouse Monoclonal antibody [C33] recognizes CD82
Tested Reactivity Hu
Tested Application FACS, FuncSt, ICC/IF, IHC-P, IP, WB
Specificity The clone C33 reacts with CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.
Host Mouse
Clonality Monoclonal
Clone C33
Isotype IgG2a
Target Name CD82
Antigen Species Human
Immunogen C91/PL (human HTLV-1+ T cell line)
Conjugation Un-conjugated
Alternate Names Tetraspanin-27; C33 antigen; R2; Tspan-27; TSPAN27; KAI1; CD antigen CD82; CD82 antigen; ST6; 4F9; Suppressor of tumorigenicity 6 protein; SAR2; IA4; Metastasis suppressor Kangai-1; Inducible membrane protein R2; GR15; C33

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
Application Note Functional studies: In human MOLT-4 T-cell line the antibody C33 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 3732 Human CD82

Swiss-port # P27701 Human CD82 antigen

Gene Symbol CD82
Gene Full Name CD82 molecule
Background CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.
Function Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. [UniProt]
Highlight Related products:
CD82 antibodies; Anti-Mouse IgG secondary antibodies;
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Research Area Cancer antibody; Gene Regulation antibody; Immune System antibody
Calculated MW 30 kDa

Images (3) Click the Picture to Zoom In

  • ARG54184 anti-CD82 antibody [C33] IHC-P image

    Immunohistochemistry: Paraffin-embedded Human tonsil tissue stained with ARG54184 anti-CD82 antibody [C33].

  • ARG54184 anti-CD82 antibody [C33] FACS image

    Flow Cytometry: Separation of human CD82 positive lymphocytes (red-filled) from CD82 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG54184 anti-CD82 antibody [C33] at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

  • ARG54184 anti-CD82 antibody [C33] FACS image

    Flow Cytometry: Human peripheral blood stained with ARG54184 anti-CD82 antibody [C33] at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.