ARG21194

anti-CD8b antibody [EP42] (Biotin)

anti-CD8b antibody [EP42] (Biotin) for Flow cytometry,IHC-Frozen sections and Chicken

Overview

Product Description Biotin-conjugated Mouse Monoclonal antibody [EP42] recognizes CD8b
Tested Reactivity Chk
Tested Application FACS, IHC-Fr
Specificity Chicken CD8β. The clone EP42 recognizes the CD8β chain.
Host Mouse
Clonality Monoclonal
Clone EP42
Isotype IgG2a, kappa
Target Name CD8b
Antigen Species Chicken
Immunogen Chicken splenocytes
Conjugation Biotin
Full Name CD8b molecule
Alternate Names LY3; CD8B1; CD antigen CD8b; LEU2; T-cell surface glycoprotein CD8 beta chain; P37; LYT3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS< 1 µg/10^6 cells
IHC-FrAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 24 kDa

Properties

Form Liquid
Buffer PBS and Sodium azide
Preservative Sodium azide
Concentration 0.5 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CD8B
Gene Full Name CD8b molecule
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010]
Function Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. [UniProt]
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CD8 antibodies; CD8 ELISA Kits; CD8 Duos / Panels; Anti-Mouse IgG secondary antibodies;
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