ARG52237

anti-Calretinin antibody

anti-Calretinin antibody for Western blot,ICC/IF,Immunohistochemistry and Rat

Neuroscience antibody

Overview

Product Description Sheep Polyclonal antibody recognizes Calretinin
Tested Reactivity Rat
Predict Reactivity Hu, Ms, NHuPrm
Tested Application ICC/IF, IHC, WB
Host Sheep
Clonality Polyclonal
Isotype IgG
Target Name Calretinin
Antigen Species Guinea pig
Immunogen Purified guinea pig calretinin
Conjugation Un-conjugated
Alternate Names CAB29; CR; CAL2; 29 kDa calbindin; Calretinin

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IHC1:500
WB1:1,000
Application Note Specific for the ~29 kDa calretinin protein in Western blots of Rat brain lysate. The antibody also works well for Immunohistochemistry on formalin or paraformaldehyde perfused Rat brain tissue sections.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein A purified
Buffer 10 mM Tris and 100 mM NaCl
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 117059 Rat CALB2

Swiss-port # P47728 Rat Calretinin

Background Calretinin is a calcium-binding protein involved in calcium signaling. It is abundantly expressed throughout the central nervous system, retina and many other tissues. Studies have shown that calretinin may play a role in neuroprotection by buffering intracellular calcium (Lukas and Jones 1994, Weisenhorn et al., 1996). Additionally, calretinin selectively stains mesothelial cells thus making it an important marker for differentiating malignant mesothelioma from carcinomas (Attanoos et al., 2000).
Highlight Related products:
Calretinin antibodies; Anti-Sheep IgG secondary antibodies;
Related news:
Neuronal Development Marker
Research Area Neuroscience antibody
Calculated MW 32 kDa

Images (2) Click the Picture to Zoom In

  • ARG52237 anti-Calretinin antibody WB image

    Western Blot: rat cerebellum (CB) lysate showing specific immunolabeling of the ~29k calretinin protein stained with Calretinin antibody (ARG52237).

  • ARG52237 anti-Calretinin antibody IHC image

    Immunohistochemistry: rat cerebellum stained with ARG52237 anti-Calretinin antibody showing fluorescence of calretinin containing cells.