anti-Calretinin antibody for Western blot,ICC/IF,Immunohistochemistry and Rat
|Product Description||Sheep Polyclonal antibody recognizes Calretinin|
|Predict Reactivity||Hu, Ms, NHuPrm|
|Tested Application||ICC/IF, IHC, WB|
|Antigen Species||Guinea pig|
|Immunogen||Purified guinea pig calretinin|
|Alternate Names||CAB29; CR; CAL2; 29 kDa calbindin; Calretinin|
|Application Note||Specific for the ~29 kDa calretinin protein in Western blots of Rat brain lysate. The antibody also works well for Immunohistochemistry on formalin or paraformaldehyde perfused Rat brain tissue sections.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Protein A purified|
|Buffer||10 mM Tris and 100 mM NaCl|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Background||Calretinin is a calcium-binding protein involved in calcium signaling. It is abundantly expressed throughout the central nervous system, retina and many other tissues. Studies have shown that calretinin may play a role in neuroprotection by buffering intracellular calcium (Lukas and Jones 1994, Weisenhorn et al., 1996). Additionally, calretinin selectively stains mesothelial cells thus making it an important marker for differentiating malignant mesothelioma from carcinomas (Attanoos et al., 2000).|
Calretinin antibodies; Anti-Sheep IgG secondary antibodies;
Neuronal Development Marker
|Research Area||Neuroscience antibody|
|Calculated MW||32 kDa|
Images (2) Click the Picture to Zoom In
ARG52237 anti-Calretinin antibody WB image
Western Blot: rat cerebellum (CB) lysate showing specific immunolabeling of the ~29k calretinin protein stained with Calretinin antibody (ARG52237).
ARG52237 anti-Calretinin antibody IHC image
Immunohistochemistry: rat cerebellum stained with ARG52237 anti-Calretinin antibody showing fluorescence of calretinin containing cells.