ARG20048

anti-Caspase 9 (cleaved) antibody

anti-Caspase 9 (cleaved) antibody for Immunoprecipitation,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
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anti-Caspase 9 (cleaved) antibody Rabbit Polyclonal antibody recognizes Caspase 9 (cleaved) ARG20048 Arigo biolaboratories
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anti-Caspase 9 (cleaved) antibody is rated 4.5 out of 5 by 2.

anti-Caspase 9 (cleaved) antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Caspase 9 (cleaved)
Tested Reactivity Hu
Tested Application IP, WB
Specificity The anti-active caspase-9 antibody recognizes only the cleaved caspase-9 (37 kDa). It does not recognize full-length caspase-9 or any other caspases.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Caspase 9 (cleaved)
Species Human
Immunogen Synthetic peptide mapping to the N-terminus adjacent to Asp330 of Human Caspase 9.
Conjugation Un-conjugated
Full Name caspase 9, apoptosis-related cysteine peptidase
Alternate Names APAF-3; ICE-LAP6; PPP1R56; CASP-9; Apoptotic protease-activating factor 3; Caspase-9; ICE-like apoptotic protease 6; Apoptotic protease Mch-6; APAF3; MCH6; EC 3.4.22.62

Application Instructions

Application Suggestion
Tested Application Dilution
IP10-20 µg/ml
WB0.5-4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 46 kDa

Properties

Form Liquid
Purification Affinity Purified Antibody
Buffer PBS, 50% Glycerol, 0.5% BSA and 0.02% Thimerosal
Preservative 0.02% Thimerosal
Stabilizer 50% Glycerol, 0.5% BSA
Concentration 0.2 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 842 Human CASP9

Swiss-port # P55211 Human Caspase-9

Gene Symbol CASP9
Gene Full Name caspase 9, apoptosis-related cysteine peptidase
Background Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase-9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase-9 (47 kDa) and Apaf-1. The complex processes pro-caspase-9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase-9 further processes other caspases including caspase-3 and caspase-6, to initiate a caspase cascade leading to apoptosis. The affinity purified antibody recognizing the active forms of caspase-9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Function Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30106 Mitochondria/Caspase Dependant Apoptosis Marker Antibody Duo (Caspase9, Cytochrome c)
ARG30110 Mitochondria/Caspase dependant Apoptosis Antibody Panel (Caspase3, Caspase9, Cytochrome c, PARP) (WB)
Related products:
Caspase 9 antibodies; Caspase 9 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
PTM Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage.

Images (1) Click the Picture to Zoom In

  • ARG20048 anti-Caspase 9 (cleaved) antibody WB image

    Western blot: 20 µg of HeLa untreated or treated with Staurosporine and stained with ARG20048 anti-Caspase 9 (cleaved) antibody at 1:500 dilution.

Customer's Feedback

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Review for anti-Caspase 9 (cleaved) antibody

Application:WB

Sample:HeLa untreated / treated with Staurosporine (1μM, over night)

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1 μg/ml

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-Caspase 9 (cleaved) antibody

Application:WB

Sample:HeLa untreated/treated with Staurosporine

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC