ARG10711

anti-Coronin 1 / TACO antibody

anti-Coronin 1 / TACO antibody for ICC/IF,Western blot and Human,Mouse,Rat,Cow,Horse,Pig

Overview

Product Description Rabbit Polyclonal antibody recognizes Coronin 1 / TACO
Tested Reactivity Hu, Ms, Rat, Cow, Hrs, Pig
Predict Reactivity Chk
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Coronin 1 / TACO
Immunogen Full length recombinant protein.
Conjugation Un-conjugated
Full Name coronin, actin binding protein, 1A
Alternate Names Clipin-A; Coronin-1A; Coronin-like protein A; CLABP; IMD8; CLIPINA; Tryptophan aspartate-containing coat protein; HCORO1; Coronin-like protein p57; TACO; p57

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:500 - 1:1000
WB1:20000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 11151 Human CORO1A

GeneID: 12721 Mouse CORO1A

GeneID: 155151 Rat CORO1A

Gene Symbol CORO1A
Gene Full Name coronin, actin binding protein, 1A
Background This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16. [provided by RefSeq, Sep 2010]
Function May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes. [UniProt]
PTM phosphorylation at Thr-412 by PKC strongly down-regulates the association with actin.
Polyubiquitinated by RNF128 with 'Lys-48'-linked chains, leading to proteasomal degradation.

Images (4) Click the Picture to Zoom In

  • ARG10711 anti-Coronin 1 / TACO antibody ICC/IF image

    Immunocytochemistry: A mixed neuron / glial culture from newborn Rat brain stained with ARG10711 anti-Coronin 1 / TACO antibody (green) at 1:10000 and co-stained with chicken antibody to vimentin at 1:5000. Blue is nuclear DNA counter stain. Glial cells and fibroblasts stain with vimentin, while microglia alone stain strongly and specifically for Coronin 1a, which can therefore be used as a robust marker of this important cell type.

  • ARG10711 anti-Coronin 1 / TACO antibody ICC/IF image

    Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG10711 anti-Coronin 1 / TACO antibody (red) at 1:1000 dilution and costained with Mouse mAb to GFAP (green) at 1:1000 dilution. Hoechst (blue) for nuclear staining.

    The Coronin 1 antibody labels protein expressed in the cytoplasm of microglia cells, while GFAP antibody stains intermediate filaments in astrocytic cells.

  • ARG10711 anti-Coronin 1 / TACO antibody WB image

    Western blot: HL60 cell extract stained with ARG10711 anti-Coronin 1 / TACO antibody at 1:10000 dilution. A prominent band running with an apparent SDS-PAGE molecular weight of ~57 kDa corresponds to Coronin 1a.

  • ARG10711 anti-Coronin 1 / TACO antibody WB image

    Western blot: Mouse brain, Rat brain, Cow cerebellum, Cow cortex and Pig spinal cord lysates stained with ARG10711 anti-Coronin 1 / TACO antibody (green) at 1:5000 dilution.