ARG53180

anti-Cytokeratin 14 antibody [SP53]

anti-Cytokeratin 14 antibody [SP53] for IHC-Formalin-fixed paraffin-embedded sections,Western blot,Flow cytometry and Human

Signaling Transduction antibody

Overview

Product Description Rabbit Monoclonal antibody [SP53] recognizes Cytokeratin 14
Tested Reactivity Hu
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SP53
Isotype IgG
Target Name Cytokeratin 14
Antigen Species Human
Immunogen Synthetic peptide corresponding to C-terminus of Human Cytokeratin 14.
Conjugation Un-conjugated
Alternate Names K14; CK-14; Cytokeratin-14; Keratin, type I cytoskeletal 14; EBS3; EBS4; NFJ; Keratin-14; CK14

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-Dependent
IHC-P1:100
WB1:25
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 30 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Prostate, Skin, Squamous Cell Carcinoma, A431

Properties

Form Liquid
Purification Purified by protein A/G
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3861 Human KRT14

Swiss-port # P02533 Human Keratin, type I cytoskeletal 14

Background Keratin 14 belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5.
Cellular Localization Cytoplasm
Highlight Related products:
Keratin14 antibodies; Anti-Rabbit IgG secondary antibodies;
Related news:
Stem cell and the regenerative medicine: Ready for the patients
Research Area Signaling Transduction antibody
Calculated MW 52 kDa
PTM A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus.
Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex.

Images (13) Click the Picture to Zoom In

  • ARG53180 anti-Cytokeratin 14 antibody [SP53] WB image

    Western blot: A431 cell lysate stained with ARG53180 anti-Cytokeratin 14 antibody [SP53].

  • ARG53180 anti-Cytokeratin 14 antibody [SP53] FACS image

    Flow Cytometry: A431 cells stained with ARG53180 anti-Cytokeratin 14 antibody [SP53] (green) compare to negative control of Rabbit IgG (blue).

  • ARG53180 anti-Cytokeratin 14 antibody [SP53] IHC-P image

    Immunohistochemistry: Human Cervical Squamous Cell Carcinoma stained with ARG53180 anti-Cytokeratin 14 antibody [SP53].

  • ARG53180 anti-Cytokeratin 14 antibody [SP53] IHC-P image

    Immunohistochemistry: Human Cervix stained with ARG53180 anti-Cytokeratin 14 antibody [SP53].

  • ARG53180 anti-Cytokeratin 14 antibody [SP53] IHC-P image

    Immunohistochemistry: Human Esophagus stained with ARG53180 anti-Cytokeratin 14 antibody [SP53].

  • ARG53180 anti-Cytokeratin 14 antibody [SP53] IHC-P image

    Immunohistochemistry: Human Ovarian Adenocarcinoma stained with ARG53180 anti-Cytokeratin 14 antibody [SP53].

  • ARG53180 anti-Cytokeratin 14 antibody [SP53] IHC-P image

    Immunohistochemistry: Human Prostate Adenocarcinoma stained with ARG53180 anti-Cytokeratin 14 antibody [SP53].

  • ARG53180 anti-Cytokeratin 14 antibody [SP53] IHC-P image

    Immunohistochemistry: Human Prostate stained with ARG53180 anti-Cytokeratin 14 antibody [SP53].

  • ARG53180 anti-Cytokeratin 14 antibody [SP53] IHC-P image

    Immunohistochemistry: Human Skin Squamous Cell Carcinoma stained with ARG53180 anti-Cytokeratin 14 antibody [SP53].

  • ARG53180 anti-Cytokeratin 14 antibody [SP53] IHC-P image

    Immunohistochemistry: Human Skin stained with ARG53180 anti-Cytokeratin 14 antibody [SP53].

  • ARG53180 anti-Cytokeratin 14 antibody [SP53] IHC-P image

    Immunohistochemistry: Human Thymus stained with ARG53180 anti-Cytokeratin 14 antibody [SP53].

  • ARG53180 anti-Cytokeratin 14 antibody [SP53] IHC-P image

    Immunohistochemistry: Human Tonsil stained with ARG53180 anti-Cytokeratin 14 antibody [SP53].

  • ARG53180 anti-Cytokeratin 14 antibody [SP53] IHC-P image

    Immunohistochemistry: Human Prostate stained with ARG53180 anti-Cytokeratin 14 antibody [SP53].