anti-Cytokeratin 19 antibody [SQab1872]
anti-Cytokeratin 19 antibody [SQab1872] for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry,ICC/IF,Immunoprecipitation and Human
|Product Description||Recombinant Rabbit Monoclonal antibody [SQab1872] recognizes Cytokeratin 19|
|Tested Application||FACS, ICC/IF, IHC-P, IP, WB|
|Target Name||Cytokeratin 19|
|Immunogen||Synthetic peptide around the N-terminus of Cytokeratin 19.|
|Alternate Names||Cytokeratin-19; K19; CK-19; K1CS; Keratin-19; CK19; Keratin, type I cytoskeletal 19|
|Application Note||IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Purification with Protein A.|
|Buffer||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.|
|Preservative||0.01% Sodium azide|
|Stabilizer||40% Glycerol and 0.05% BSA|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||keratin 19, type I|
|Background||Cytokeratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]|
|Function||Cytokeratin 19 involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt]|
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|Calculated MW||44 kDa|
Images (5) Click the Picture to Zoom In
ARG66324 anti-Cytokeratin 19 antibody [SQab1872] ICC/IF image
Immunofluorescence: MCF-7 cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66324 anti-Cytokeratin 19 antibody [SQab1872] (green) at 1:40,000 and 4°C. DAPI (blue) was used as the nuclear counter stain.
ARG66324 anti-Cytokeratin 19 antibody [SQab1872] FACS image
Flow Cytometry: MCF-7 cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66324 anti-Cytokeratin 19 antibody [SQab1872] (red) at 1:200 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control. .
ARG66324 anti-Cytokeratin 19 antibody [SQab1872] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded thyroid cancer tissue stained with ARG66324 anti-Cytokeratin 19 antibody [SQab1872] at 1:40,000 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
ARG66324 anti-Cytokeratin 19 antibody [SQab1872] WB image
Western blot: 10 µg of MCF7, Caco-2, HaCat and PC-3 cell lysates stained with ARG66324 anti-Cytokeratin 19 antibody [SQab1872] at 1:1000 dilution.
ARG66324 anti-Cytokeratin 19 antibody [SQab1872] IP image
Immunoprecipitation: 0.4 mg of HaCat whole cell lysate immunoprecipitated (1:100) and stained with ARG66324 anti-Cytokeratin 19 antibody [SQab1872]. 1) ARG66324 IP in HaCat whole cell lysate, 2) PBS instead of ARG66324 in HaCat whole cell lysate, and 3) HaCat whole cell lysate, 10 µg (input).