ARG66324

anti-Cytokeratin 19 antibody [SQab1872]

anti-Cytokeratin 19 antibody [SQab1872] for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry,ICC/IF,Immunoprecipitation and Human

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab1872] recognizes Cytokeratin 19
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1872
Isotype IgG
Target Name Cytokeratin 19
Antigen Species Human
Immunogen Synthetic peptide around the N-terminus of Cytokeratin 19.
Conjugation Un-conjugated
Full Name keratin 19, type I
Alternate Names Cytokeratin-19; K19; CK-19; K1CS; Keratin-19; CK19; Keratin, type I cytoskeletal 19

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:200 - 1:1000
ICC/IF1:20000 - 1:40000
IHC-P1:20000 - 1:40000
IP1:200
WB1:1000 - 1:2000
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 44 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3880 Human KRT19

Swiss-port # P08727 Human Keratin, type I cytoskeletal 19

Gene Symbol KRT19
Gene Full Name keratin 19, type I
Background The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]
Function Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt]
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Images (5) Click the Picture to Zoom In

  • ARG66324 anti-Cytokeratin 19 antibody [SQab1872] ICC/IF image

    Immunofluorescence: MCF-7 cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66324 anti-Cytokeratin 19 antibody [SQab1872] (green) at 1:40,000 and 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG66324 anti-Cytokeratin 19 antibody [SQab1872] FACS image

    Flow Cytometry: MCF-7 cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66324 anti-Cytokeratin 19 antibody [SQab1872] (red) at 1:200 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control. .

  • ARG66324 anti-Cytokeratin 19 antibody [SQab1872] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded thyroid cancer tissue stained with ARG66324 anti-Cytokeratin 19 antibody [SQab1872] at 1:40,000 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.

  • ARG66324 anti-Cytokeratin 19 antibody [SQab1872] WB image

    Western blot: 10 µg of MCF7, Caco-2, HaCat and PC-3 cell lysates stained with ARG66324 anti-Cytokeratin 19 antibody [SQab1872] at 1:1000 dilution.

  • ARG66324 anti-Cytokeratin 19 antibody [SQab1872] IP image

    Immunoprecipitation: 0.4 mg of HaCat whole cell lysate immunoprecipitated (1:100) and stained with ARG66324 anti-Cytokeratin 19 antibody [SQab1872]. 1) ARG66324 IP in HaCat whole cell lysate, 2) PBS instead of ARG66324 in HaCat whole cell lysate, and 3) HaCat whole cell lysate, 10 µg (input).