ARG62969

anti-Cytokeratin (pan) antibody [C-11]

anti-Cytokeratin (pan) antibody [C-11] for CyTOF®-candidate,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mammal,Mouse,Rat

Signaling Transduction antibody; Carcinoma Marker antibody
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Overview

Product Description Mouse Monoclonal antibody [C-11] recognizes Cytokeratin (pan)
Tested Reactivity Hu, Ms, Rat, Mamm
Tested Application CyTOF®-candidate, FACS, ICC/IF, IHC-P, IP, WB
Specificity The clone C-11 reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Host Mouse
Clonality Monoclonal
Clone C-11
Isotype IgG1
Target Name Cytokeratin (pan)
Antigen Species Human
Immunogen Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
Conjugation Un-conjugated
Full Name keratin 1, type II
Alternate Names 67 kDa cytokeratin; KRT1A; EPPK; Keratin, type II cytoskeletal 1; EHK1; K1; Keratin-1; CK1; Cytokeratin-1; Hair alpha protein; Type-II keratin Kb1; CK-1; NEPPK; EHK

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS0.5 µg/ml
ICC/IFAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 16678 Mouse KRT1

GeneID: 300250 Rat KRT1

GeneID: 3848 Human KRT1

Gene Symbol KRT1
Gene Full Name keratin 1, type II
Background Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Function May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30319 Carcinoma / Sarcoma Antibody Duo
Related products:
Cytokeratin antibodies; Cytokeratin Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Signaling Transduction antibody; Carcinoma Marker antibody
Calculated MW 66 kDa
PTM Undergoes deimination of some arginine residues (citrullination).

Images (3) Click the Picture to Zoom In

  • ARG62969 anti-Cytokeratin (pan) antibody [C-11] WB image

    Western blot: 30 µg of MCF-7 cell lysate stained with ARG62969 anti-Cytokeratin (pan) antibody [C-11] at 1:500 dilution.

  • ARG62969 anti-Cytokeratin (pan) antibody [C-11] ICC/IF image

    Immunofluorescence: Rat endometrial epithelial (REE) cultured cells fixed with neutral buffered formalin for 10 min, and permeabilized with cold-methanol at -20°C for 10 min and then stained with ARG62969 anti-Cytokeratin (pan) antibody [C-11].

  • ARG62969 anti-Cytokeratin (pan) antibody [C-11] IHC-P image

    Immunohistochemistry: Paraffin-embedded sections of guinea pig breast carcinoma stained with ARG62969 anti-Cytokeratin (pan) antibody [C-11].

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Review for anti-Cytokeratin (pan) antibody [C-11]

Application:WB

Sample:Mouse kidney, Rat kidney

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Clone References

Intracellular distribution of p300 and its differential recruitment to aggresomes in breast cancer.

Fermento ME et al.
Exp Mol Pathol.,  (2010)

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Establishment and characterization of baboon embryonic stem cell lines: an Old World Primate model for regeneration and transplantation research.

ICC/IF / Baboon

Simerly CR et al.
Stem Cell Res.,  (2009)

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