ARG52283

anti-FOX3 / NeuN antibody [1B7]

anti-FOX3 / NeuN antibody [1B7] for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Bovine,Cat,Chicken,Dog,Goat,Pig

Controls and Markers antibody; Gene Regulation antibody; Neuroscience antibody
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Overview

Product Description

Mouse Monoclonal antibody [1B7] recognizes FOX3 / NeuN

Tested Reactivity Hu, Ms, Rat, Bov, Cat, Chk, Dog, Goat, Pig
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 1B7
Isotype IgG2a
Target Name FOX3 / NeuN
Antigen Species Human
Immunogen Recombinant protein taken from the N-terminus of human FOX3 expressed in and purified from E. Coli
Conjugation Un-conjugated
Alternate Names RNA binding protein fox-1 homolog 3; NEUN; FOX-3; HRNBP3; Fox-1 homolog C; FOX3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200 - 1:1000
IHC-Fr1:200 - 1:1000
IHC-P1:200 - 1:1000
WB1:1000 - 1:2000
Application Note Specific for the ~46/48k FOX3 protein doublet
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer PBS and 10 mM Sodium azide.
Preservative 10 mM Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 146713 Human RBFOX3

GeneID: 511773 Bovine RBFOX3

GeneID: 52897 Mouse RBFOX3

Gene Symbol RBFOX3
Gene Full Name RNA binding protein, fox-1 homolog (C. elegans) 3
Background FOX3, also known as NeuN and hexaribonucleotide binding protein 3, is a neuron-specific RNA binding nuclear protein involved in the regulation of pre-mRNA alternative splicing (Kim et al., 2009). FOX3 dependent alternative splicing of Numb has recently been shown to play an important role in the progression of neuronal differentiation during vertebrate development (Kim et al., 2013).
Highlight Related products:
FOX3 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
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Neuronal Development Marker
Research Area Controls and Markers antibody; Gene Regulation antibody; Neuroscience antibody
Calculated MW 34 kDa

Images (5) Click the Picture to Zoom In

  • ARG52283 anti-FOX3 / NeuN antibody [1B7] ICC/IF image

    Immunofluorescence: Cultured Rat neurons stained with ARG52283 anti-FOX3 / NeuN antibody [1B7] showing strong nuclear and distal cytoplasmic staining of FOX3 in red and the complete absence of astrocyte staining, which are stained in green with ARG52313 anti-GFAP antibody.

  • ARG52283 anti-FOX3 / NeuN antibody [1B7] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Rat brain stem neurons stained with ARG52283 anti-FOX3 / NeuN antibody [1B7] (green) and counter stained with anti-MAP2 antibody (red). The nuclei of cells are revealed with DAPI in blue.

  • ARG52283 anti-FOX3 / NeuN antibody [1B7] WB image

    Western Blot: Rat cortex lysate showing specific immunolabeling of the ~46/48k FOX3 protein stained with ARG52283 anti-FOX3 / NeuN antibody [1B7].

  • ARG52283 anti-FOX3 / NeuN antibody [1B7] WB image

    Western blot: Rat brain, Rat brain (embryonic, E20) and Mouse brain lysates stained with ARG52283 anti-FOX3 / NeuN antibody [1B7] (green) at 1:1000 dilution.

  • ARG52283 anti-FOX3 / NeuN antibody [1B7] IHC-FoFr image

    Immunohistochemistry: Adult Rat hippocampus stained with anti-c-Fos antibody (red) and DNA (blue). ARG52283 anti-FOX3 / NeuN antibody [1B7] (green) reveals strong nuclear and distal cytoplasmic staining for hippocampal neurons and the complete absence of staining of nonneuronal cells.

    A few spontaneously active cells show in addition expression of c-Fos (arrowed). ARG52283 is therefore and excellent marker of neuronal activation.

Customer's Feedback

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Review for anti-FOX3 / NeuN antibody [1B7]

Application:WB

Sample:Mouse brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Mesenchymal Stem Cell-Derived Exosomes Ameliorate Alzheimer's Disease Pathology and Improve Cognitive Deficits.

WB / Human

Yi-An Chen et al.
Biomedicines.,  (2021)

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Ghrelin fiber projections from the hypothalamic arcuate nucleus into the dorsal vagal complex and the regulation of glycolipid metabolism.

IHC-Fr / Rat

Su Manqing et al.
Neuropeptides.,  (2019)

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Evolutional Characterization of Photochemically Induced Stroke in Rats: a Multimodality Imaging and Molecular Biological Study.

IHC-P / Rat

Liu NW et al.
Transl Stroke Res.,  (2016)

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