ARG51585

anti-G3BP1 phospho (Ser232) antibody

anti-G3BP1 phospho (Ser232) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human

Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes G3BP1 phospho (Ser232)
Tested Reactivity Hu
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name G3BP1
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of serine 232 (S-S-S(p)-P-A) derived from Human G3BP-1.
Conjugation Un-conjugated
Alternate Names G3BP; hDH VIII; G3BP-1; GAP SH3 domain-binding protein 1; EC 3.6.4.13; EC 3.6.4.12; HDH-VIII; ATP-dependent DNA helicase VIII; Ras GTPase-activating protein-binding protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10146 Human G3BP1

Swiss-port # Q13283 Human Ras GTPase-activating protein-binding protein 1

Gene Symbol G3BP1
Gene Full Name GTPase activating protein (SH3 domain) binding protein 1
Background May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
Function May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. [UniProt]
Research Area Gene Regulation antibody
Calculated MW 52 kDa
PTM Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
Arg-435 is dimethylated, probably to asymmetric dimethylarginine.

Images (3) Click the Picture to Zoom In

  • ARG51585 anti-G3BP1 phospho (Ser232) antibody WB image

    Western blot: Extracts from 293 cells untreated or treated with starvation stained with ARG51585 anti-G3BP1 phospho (Ser232) antibody.

  • ARG51585 anti-G3BP1 phospho (Ser232) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51585 anti-G3BP1 phospho (Ser232) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51585 anti-G3BP1 phospho (Ser232) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with ARG51585 anti-G3BP1 phospho (Ser232) antibody.