ARG46751

anti-GCLM antibody

anti-GCLM antibody for ICC/IF,Western blot,Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes GCLM
Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GCLM
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide between 246-274 amino acids from the C-terminal region of Human IREB2 / IRP2
Conjugation Un-conjugated
Alternate Names GCS light chain; Gamma-ECS regulatory subunit; Glutamate--cysteine ligase regulatory subunit; GLCLR; Gamma-glutamylcysteine synthetase regulatory subunit; Glutamate--cysteine ligase modifier subunit

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10-1:50
ICC/IF1:10-1:50
IHC-P~1:25
WB~1:8000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen
Purification Note Abcepta (Abgent)
Purity AP9102B-ev
Buffer PBS and 0.09% sodium azide
Preservative 0.09% sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14630 Mouse GCLM

GeneID: 2730 Human GCLM

Swiss-port # O09172 Mouse Glutamate--cysteine ligase regulatory subunit

Swiss-port # P48507 Human Glutamate--cysteine ligase regulatory subunit

Gene Symbol GCLM
Gene Full Name glutamate-cysteine ligase, modifier subunit
Background Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]
Calculated MW 31 kDa