ARG10755

anti-GFP antibody

anti-GFP antibody for ICC/IF,IHC-Frozen sections,Western blot and Other

Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody

Overview

Product Description

Chicken Polyclonal Antibody to GFP

Tested Reactivity Other
Tested Application ICC/IF, IHC-Fr, WB
Host Chicken
Clonality Polyclonal
Isotype IgY
Target Name GFP
Species Others
Immunogen Prot-aceGFP recombinant protein.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000 - 1:5000
IHC-Fr1:1000 - 1:5000
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Research Area Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody

Images (4) Click the Picture to Zoom In

  • ARG10755 anti-GFP antibody ICC/IF image

    Immunocytochemistry: Transfected Hek293 cells which overexpressed GFP-fusion protein fused to a nuclear localization sequence  were stained with ARG10755 anti-GFP antibody (red). Most Hek293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with GFP are bright green. Red antibody staining is only seen in cells which express GFP, as expected, and the superimposition of green and red results in an orange signal.

  • ARG10755 anti-GFP antibody ICC/IF image

    Immunofluorescence: Transfected HEK293 cells with a GFP fusion protein construct (green), and stained with ARG10755 anti-GFP antibody (red) at 1:1000 dilution. DAPI (blue) for nuclear staining.

    The GFP antibody reveals GFP protein expressed only in transfected cells, and as a result these cells appear golden yellow in color. Top, merged image, bottom individual channels.

  • ARG10755 anti-GFP antibody WB image

    Western blot: HEK293 cells transfected with pFin-EF1-GFP vector. This vector expresses full length GFP and crude homogenate from these cells was stained with ARG10755 anti-GFP antibody.

  • ARG10755 anti-GFP antibody WB image

    Western blot: 1) HEK293 (non-transfected), 2) HEK293 transfected with a GFP construct and 3) HEK293 transfected with an mCherry construct. The blots were stained with ARG10755 anti-GFP antibody (green) at 1:1000 dilution. The blot was simultaneously stained with ARG10757 anti-Hsp 60 antibody (red) at 1:10000 dilution.