anti-GluR1 Subunit phospho (Ser831) antibody

anti-GluR1 Subunit phospho (Ser831) antibody for Western blot and Rat

Neuroscience antibody; Postsynaptic Receptor antibody


Product Description Rabbit Polyclonal antibody recognizes GluR1 Subunit phospho (Ser831)
Tested Reactivity Rat
Predict Reactivity Hu, Ms, Chk, Dog
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GluR1 Subunit
Antigen Species Rat
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser831 conjugated to KLH
Conjugation Un-conjugated
Alternate Names GLUH1; GluA1; GluR-1; Glutamate receptor ionotropic, AMPA 1; GluR-K1; GLUR1; HBGR1; AMPA-selective glutamate receptor 1; GluR-A; GLURA; Glutamate receptor 1

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note Specific for the ~100k GluR1 protein phosphorylated at Ser831. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is completely eliminated by λ-phosphatase treatment.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 50592 Rat GRIA1

Swiss-port # P19490 Rat Glutamate receptor 1

Gene Symbol GRIA1
Gene Full Name glutamate receptor, ionotropic, AMPA 1
Background The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is potentiated by phosphorylation at Ser831 which has been shown to be mediated by either PKC or CaM kinase II (McGlade-McCulloh et al., 1993; Mammen et al., 1999; Roche et al., 1996). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Soderling and Derkach, 2000).
Highlight Related Antibody Duos and Panels:
ARG30132 Phospho GluR1 Antibody Panel
Related products:
GluR1 antibodies; GluR1 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Neuroscience antibody; Postsynaptic Receptor antibody
Calculated MW 102 kDa
PTM Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).
Phosphorylated at Ser-645. Phosphorylated at Ser-710 by PKC. Phosphorylated at Ser-849 by PKC, PKA and CAMK2. Phosphorylated at Ser-863 by PKC, PKA and PRKG2.

Images (1) Click the Picture to Zoom In

  • ARG52315 anti-GluR1 Subunit phospho (Ser831) antibody WB image

    Western blot: Rat hippocampal lysate showing phospho-specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser831 stained with ARG52315 anti-GluR1 Subunit phospho (Ser831) antibody.