ARG10109

anti-HLA G antibody [MEM-G/2]

anti-HLA G antibody [MEM-G/2] for IHC-Formalin-fixed paraffin-embedded sections,Western blot,IHC-Frozen sections and Human

Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [MEM-G/2] recognizes HLA G
Tested Reactivity Hu
Tested Application IHC-Fr, IHC-P, WB
Specificity The clone MEM-G/2 recognizes the free heavy chain of all the HLA-G isoforms. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
Host Mouse
Clonality Monoclonal
Clone MEM-G/2
Isotype IgG1
Target Name HLA G
Immunogen Bacterially expressed recombinant HLA-G heavy chain (denatured).
Conjugation Un-conjugated
Full Name major histocompatibility complex, class I, G
Alternate Names HLA G antigen; MHC class I antigen G; HLA class I histocompatibility antigen, alpha chain G; MHC-G

Application Instructions

Application Note
Immunohistochemistry (paraffin sections):
Western Blotting:
Immunohistochemistry (frozen sections):
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by hydrophobic charge induction chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3135 Human HLA-G

Swiss-port # P17693 Human HLA class I histocompatibility antigen, alpha chain G

Gene Symbol HLA-G
Gene Full Name major histocompatibility complex, class I, G
Background HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]
Function Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells. [UniProt]
Research Area Immune System antibody

(1) Click the Picture to Zoom In

  • ARG10109 anti-HLA G antibody [MEM-G/2] IHC-P image

    Immunohistochemistry: Paraffin-embedded kidney allograft biopsy stained with ARG10109 anti-HLA G antibody [MEM-G/2].

Clone References

Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions.

WB / Human

Singleton DR et al.
J Cell Sci.,  (1997)

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