anti-IL1 beta antibody

anti-IL1 beta antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunohistochemistry (PFA perfusion fixed frozen sections),Neutralizing,Western blot and Mouse,Rat

Pyroptosis Study antibody
publication_link Publication3


Product Description Rabbit Polyclonal antibody recognizes IL1 beta 
Tested Reactivity Ms, Rat
Tested Application ELISA, ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, Neut, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IL1 beta
Antigen Species Mouse
Immunogen E.coli derived Recombinant Mouse IL-1β.
Conjugation Un-conjugated
Alternate Names Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56754 as a detection antibody
ICC/IFAssay dependent
IHC-FoFr Assay dependent
IHC-Fr0.5 µg/ml
IHC-PAssay dependent
Neut0.57 - 0.86 µg/ml (To yield [ND50] of the biological activity of mIL - 1β (10 pg/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 16176 Mouse IL1B

Swiss-port # P10749 Mouse Interleukin-1 beta

Gene Symbol Il1b
Gene Full Name interleukin 1 beta
Background IL1 beta protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
Function IL1 beta is a potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). [UniProt]
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Research Area Pyroptosis Study antibody
Calculated MW 31 kDa
PTM Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.

Images (3) Click the Picture to Zoom In

  • ARG56644 anti-IL1 beta antibody WB image

    Western blot: 250 - 0.24 ng of Mouse IL-1β stained with ARG56644 anti-IL1 beta antibody, under reducing conditions.

  • ARG56644 anti-IL1 beta antibody WB image

    Western blot: 250 - 0.24 ng of Mouse IL-1β stained with ARG56644 anti-IL1 beta antibody, under non-reducing conditions.

  • ARG56644 anti-IL1 beta antibody standard curve image

    Sandwich ELISA: ARG56644 anti-IL1 beta antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56754 anti-IL1 beta antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.

Specific References

Protective role of thymoquinone in hyperlipidemia-induced liver injury in LDL-R-/-mice

WB / Mouse

Fei Wang et al.
BMC Gastroenterol,  (2023)




Osteopontin N-Terminal Function in an Abdominal Aortic Aneurysm From Apolipoprotein E-Deficient Mice

WB, ICC-IF, IHC-P / Mouse

Hongyang Liu et al.
Front Cell Dev Biol.,  (2021)




Thymoquinone Protects against Hyperlipidemia-Induced Cardiac Damage in Low-Density Lipoprotein Receptor-Deficient (LDL-R -/-) Mice via Its Anti-inflammatory and Antipyroptotic Effects.

WB / Mouse

Zuo-Wei Pei et al.
Biomed Res Int.,  (2020)