ARG10010

anti-IL1 beta antibody [S1H12]

anti-IL1 beta antibody [S1H12] for ELISA,Western blot and Human

Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody

Overview

Product Description

Mouse Monoclonal antibody [S1H12] recognizes IL1 beta

Tested Reactivity Hu
Tested Application ELISA, WB
Specificity Does not show any cross reaction with recombinant human IL-1α, recombinant murine IL-1α or IL-1β.
Host Mouse
Clonality Monoclonal
Clone S1H12
Isotype IgG1,k
Target Name IL1 beta
Antigen Species Human
Immunogen Purified recombinant human IL-1β
Conjugation Un-conjugated
Full Name interleukin 1, beta
Alternate Names Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2

Application Instructions

Application Note ELISA: React with human IL-1β

Western Blot: This antibody, when used at concentration of 20-100 ng/mL will allow visualization of 100 ng/lane of human IL-1β.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2)
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3553 Human IL1B

Swiss-port # P01584 Human Interleukin-1 beta

Gene Symbol IL1B
Gene Full Name interleukin 1, beta
Background Interleukin 1β (IL-1β) shares the IL-1 receptor with other cytokines in IL-1 family. It is primarily produced by activated macrophage as a proprotein, which is cleaved by caspase I to become active. Like IL-1α, IL-1β is an important mediator of innate immunity. IL-1β stimulates lymphocyte proliferation and the production of TNF-α, IL-6 and IL-8. IL-1β and TNF-α can synergically act at NFkB and mitogen kinases including JNKs and p38 MARKs, thus leading to the selective expression of genes for immunity and inflammation.
Function Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody
PTM Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.