ARG10010

anti-IL1 beta antibody [S1H12]

anti-IL1 beta antibody [S1H12] for ELISA,Western blot and Human

Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody

Overview

Product Description

Mouse Monoclonal antibody [S1H12] recognizes IL1 beta

Tested Reactivity Hu
Tested Application ELISA, WB
Specificity Does not show any cross reaction with recombinant human IL-1α, recombinant murine IL-1α or IL-1β.
Host Mouse
Clonality Monoclonal
Clone S1H12
Isotype IgG1,k
Target Name IL1 beta
Antigen Species Human
Immunogen Purified recombinant human IL-1β
Conjugation Un-conjugated
Full Name interleukin 1, beta
Alternate Names Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
WB20 - 100 ng/ml
Application Note ELISA: React with human IL-1β.
WB: This antibody, when used at concentration of 20-100 ng/ml will allow visualization of 100 ng/lane of human IL-1β.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 31 kDa

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2)
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3553 Human IL1B

Swiss-port # P01584 Human Interleukin-1 beta

Gene Symbol IL1B
Gene Full Name interleukin 1, beta
Background IL1 beta protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
Function IL1 beta is a potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). [UniProt]
Research Area Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody
PTM Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.