ARG10521

anti-IL6 antibody [1B6]

anti-IL6 antibody [1B6] for ELISA,Western blot,Flow cytometry and Human

Cancer antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody

Overview

Product Description Mouse Monoclonal antibody [1B6] recognizes IL6
Tested Reactivity Hu
Tested Application ELISA, FACS, WB
Host Mouse
Clonality Monoclonal
Clone 1B6
Isotype IgG1 Kappa
Target Name IL6
Antigen Species Human
Immunogen Purified recombinant Human IL-6 (NP_000591.1)
Conjugation Un-conjugated
Full Name interleukin 6
Alternate Names B-cell stimulatory factor 2; CDF; HSF; BSF-2; Interferon beta-2; IL-6; IFNB2; CTL differentiation factor; Interleukin-6; HGF; Hybridoma growth factor; BSF2; IFN-beta-2

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS10 μg/ml
WB200 ng/ml, can recognize 25ng/lane antigen
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer 0.01M PBS (pH 7.0)
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3569 Human IL6

Swiss-port # P05231 Human Interleukin-6

Gene Symbol IL6
Gene Full Name interleukin 6
Background This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]
Function Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. [UniProt]
Research Area Cancer antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody
PTM N- and O-glycosylated.

Images (2) Click the Picture to Zoom In

  • ARG10521 anti-IL6 antibody [1B6] WB image

    Western blot: (Lane 1, 2) 25 ng of recombinant human IL-6 and (Lane 3, 4) 50 ng of recombinant human IL-6 stained with ARG10521 anti-IL6 antibody [1B6] at 200 ng/ml dilution.

  • ARG10521 anti-IL6 antibody [1B6] FACS image

    Flow Cytometry: IL-6 transfected 293T cells stained with ARG10521 anti-IL6 antibody [1B6] at 10 ug/ml dilution. Black line: stained with isotype control.