ARG45465
anti-LARG antibody
anti-LARG antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes LARG |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | LARG |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human LARG. |
| Conjugation | Un-conjugated |
| Alternate Names | ARHGEF12; Rho Guanine Nucleotide Exchange Factor 12; LARG; KIAA0382; Rho Guanine Nucleotide Exchange Factor (GEF) 12; Leukemia-Associated RhoGEF; Leukemia-Associated Rho Guanine Nucleotide Exchange Factor; KMT2A/ARHGEF12 Fusion Protein; PRO2792 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 220 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8R4H2 Mouse Rho guanine nucleotide exchange factor 12 Swiss-port # Q9NZN5 Human Rho guanine nucleotide exchange factor 12 |
|---|---|
| Gene Symbol | ARHGEF12 |
| Gene Full Name | Rho Guanine Nucleotide Exchange Factor 12 |
| Background | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014] |
| Function | May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. [UniProt] |
| Cellular Localization | Cytoplasm; Membrane. [UniProt] |
| Calculated MW | 173 kDa |
| PTM | Acetylation; Phosphoprotein. [UniProt] |
Images (7) Click the Picture to Zoom In
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ARG45465 anti-LARG antibody IHC-P image
Immunohistochemistry: Human colorectal adenocarcinoma stained with ARG45465 anti-LARG antibody at 2 μg/ml dilution.
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ARG45465 anti-LARG antibody ICC/IF image
Immunofluorescence: A549 stained with ARG45465 anti-LARG antibody at 5 μg/ml dilution.
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ARG45465 anti-LARG antibody WB image
Western blot: Hela stained with ARG45465 anti-LARG antibody at 0.5 μg/ml dilution.
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ARG45465 anti-LARG antibody FACS image
Flow Cytometry: HepG2 stained with ARG45465 anti-LARG antibody at 1 µg/10^6 cells dilution.
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ARG45465 anti-LARG antibody IHC-P image
Immunohistochemistry: Rat brain stained with ARG45465 anti-LARG antibody at 2 μg/ml dilution.
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ARG45465 anti-LARG antibody WB image
Western blot: Rat testis stained with ARG45465 anti-LARG antibody at 0.5 μg/ml dilution.
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ARG45465 anti-LARG antibody WB image
Western blot: Mouse testis stained with ARG45465 anti-LARG antibody at 0.5 μg/ml dilution.
