anti-LC3B antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Autophagy Study antibody
|Product Description||Rabbit Polyclonal antibody recognizes LC3B|
|Tested Reactivity||Hu, Ms, Rat|
|Tested Application||ICC/IF, IHC-P, WB|
|Immunogen||Synthetic peptide of Human LC3B.|
|Alternate Names||Microtubule-associated proteins 1A/1B light chain 3B; MAP1A/MAP1B light chain 3 B; MAP1A/1BLC3; MAP1 light chain 3-like protein 2; Autophagy-related protein LC3 B; MAP1A/MAP1B LC3 B; LC3B; MAP1LC3B-a; ATG8F; Microtubule-associated protein 1 light chain 3 beta; Autophagy-related ubiquitin-like modifier LC3 B|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Buffer||PBS (pH 7.3), 0.02% Sodium azide, 50% Glycerol and 0.05% BSA.|
|Preservative||0.02% Sodium azide|
|Stabilizer||50% Glycerol and 0.05% BSA|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||microtubule-associated protein 1 light chain 3 beta|
|Background||LC3B is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]|
|Function||LC3B is an Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. Through its interaction with the reticulophagy receptor TEX264, paticipates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538, PubMed:31006537). [UniProt]|
|Highlight||Related Antibody Duos and Panels:
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ARG30321 Autophagy Antibody Panel
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|Research Area||Autophagy Study antibody|
|Calculated MW||15 kDa|
|PTM||The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II (PubMed:15187094).
The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.
Phosphorylation at Thr-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE) (By similarity). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.
Images (5) Click the Picture to Zoom In
ARG55799 anti-LC3B antibody WB image (Customer's Feedback)
Western blot: HeLa cells untreated or treated with Chloroquine. 20 µg of cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody and ARG55799 anti-LC3B antibody at 1:1000 dilution.
An anti-GAPDH antibody used as a positive control.
ARG55799 anti-LC3B antibody ICC/IF image
Immunofluorescence: NIH/3T3 cells treated with Chloroquine (50 μM, 37°C for 20 hours). Cells were stained with ARG55799 anti-LC3B antibody at 1:100 dilution.
ARG55799 anti-LC3B antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney stained with ARG55799 anti-LC3B antibody at 1:100 dilution.
ARG55799 anti-LC3B antibody WB image
Western blot: 30 µg of HeLa untreated or treated with Chloroquine at 50 µM, 100 µM (18 hr). The bolts stained with ARG55799 anti-LC3B antibody at 1:1000 dilution.
ARG55799 anti-LC3B antibody WB image
Western blot: 30 µg of SW620, Mouse spleen, and Rat spleen lysates stained with ARG55799 anti-LC3B antibody at 1:1000 dilution.