ARG57581

anti-ME1 antibody

anti-ME1 antibody for Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes ME1
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ME1
Antigen Species Human
Immunogen Recombinant protein of Human ME1.
Conjugation Un-conjugated
Alternate Names NADP-dependent malic enzyme; Malic enzyme 1; HUMNDME; EC 1.1.1.40; NADP-ME; MES

Application Instructions

Predict Reactivity Note Rat
Application Suggestion
Tested Application Dilution
WB1:1000 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control LO2
Observed Size 64 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17436 Mouse ME1

GeneID: 4199 Human ME1

Swiss-port # P06801 Mouse NADP-dependent malic enzyme

Swiss-port # P48163 Human NADP-dependent malic enzyme

Gene Symbol ME1
Gene Full Name malic enzyme 1, NADP(+)-dependent, cytosolic
Background This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008]
Cellular Localization Cytoplasm
Calculated MW Isoform 1: 64 kDa
Isoform 2: 55 kDa

Images (1) Click the Picture to Zoom In

  • ARG57581 anti-ME1 antibody WB image

    Western blot: 25 µg of LO2 cell lysate stained with ARG57581 anti-ME1 antibody at 1:1000 dilution.