ARG63504
anti-MID2 / TRIM1 antibody
anti-MID2 / TRIM1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Gene Regulation antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes MID2 / TRIM1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Cow, Dog, Pig |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise both human isoforms of this protein. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MID2 / TRIM1 |
| Immunogen | QESPYVSGMKTCH |
| Conjugation | Un-conjugated |
| Alternate Names | RNF60; FXY2; RING finger protein 60; Probable E3 ubiquitin-protein ligase MID2; EC 6.3.2.-; Midline defect 2; MRX101; Midline-2; TRIM1; Tripartite motif-containing protein 1; Midin-2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9QUS6 Mouse Probable E3 ubiquitin-protein ligase MID2 Swiss-port # Q9UJV3 Human Probable E3 ubiquitin-protein ligase MID2 |
|---|---|
| Background | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009] |
| Research Area | Gene Regulation antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 83 kDa |
| PTM | Phosphorylated on serine and threonine residues. |
Images (3) Click the Picture to Zoom In
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ARG63504 anti-MID2 / TRIM1 antibody WB image
Western Blot: mouse heart lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63504 anti-MID2 / TRIM1 antibody at 1 µg/ml dilution.
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ARG63504 anti-MID2 / TRIM1 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Kidney. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63504 anti-MID2 / TRIM1 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63504 anti-MID2 / TRIM1 antibody WB image
Western blot: 35 µg of Mouse heart (A), Rat heart (B) and Rat kidney (C) lysates (in RIPA buffer) stained with ARG63504 anti-MID2 / TRIM1 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
