ARG66878
anti-NSE / Neuron Specific Enolase antibody [SQab22281]
anti-NSE / Neuron Specific Enolase antibody [SQab22281] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [SQab22281] recognizes NSE / Neuron Specific Enolase |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SQab22281 |
| Isotype | IgG |
| Target Name | NSE / Neuron Specific Enolase |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within Human NSE aa 416-433. |
| Conjugation | Un-conjugated |
| Alternate Names | Neural enolase; NSE; Enolase 2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; HEL-S-279; Neuron-specific enolase; EC 4.2.1.11 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | 47 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ENO2 |
| Gene Full Name | enolase 2 (gamma, neuronal) |
| Background | This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008] |
| Function | Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). [UniProt] |
| Highlight | Related products: NSE antibodies; Anti-Mouse IgG secondary antibodies; Related news: Neuronal Development Marker |
| Research Area | Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 47 kDa |
Images (3) Click the Picture to Zoom In
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ARG66878 anti-NSE antibody [SQab22281] IHC-P image
Immunohistochemistry: Paraffin-embedded Neuroblastoma stained with ARG66878 anti-NSE antibody [SQab22281] at 1:200.
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ARG66878 anti-NSE / Neuron Specific Enolase antibody [SQab22281] WB image (Customer review)
Western blot: HepG2 stained with ARG66878 anti-NSE / Neuron Specific Enolase antibody [SQab22281] at 1:500 dilution.
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ARG66878 anti-NSE / Neuron Specific Enolase antibody [SQab22281] WB image (Customer review)
Western blot: Mouse brain stained with ARG66878 anti-NSE / Neuron Specific Enolase antibody [SQab22281] at 1:500 dilution.
Customer's Feedback
Excellent
Review for anti-NSE / Neuron Specific Enolase antibody [SQab22281]
Application：WB
Sample：Mouse Brain
Sample Loading Amount：10 μL
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-NSE / Neuron Specific Enolase antibody [SQab22281]
Application：WB
Sample：HepG2
Sample Loading Amount：10 μL
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
