ARG10579

anti-Nephrin antibody [Y17-R]

anti-Nephrin antibody [Y17-R] for ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description

Rabbit Monoclonal antibody [Y17-R] recognizes Nephrin

Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Monoclonal
Clone Y17-R
Isotype IgG
Target Name Nephrin
Antigen Species Human
Immunogen Synthetic peptide around the center of the fifth Ig-like domain of Human nephrin (between residues Cys465-Cys528)
Conjugation Un-conjugated
Full Name nephrosis 1, congenital, Finnish type (nephrin)
Alternate Names CNF; NPHN; Renal glomerulus-specific cell adhesion receptor; nephrin; Nephrin

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 20 mM Tris-HCl (pH 8.0), 0.05% Sodium azide and 10 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 10 mg/ml BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 4868 Human NPHS1

GeneID: 54631 Mouse NPHS1

GeneID: 64563 Rat NPHS1

Gene Symbol NPHS1
Gene Full Name nephrosis 1, congenital, Finnish type (nephrin)
Background This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]
Function Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity). [UniProt]
PTM Phosphorylated at Tyr-1193 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1.

Images (2) Click the Picture to Zoom In

  • ARG10579 anti-Nephrin antibody [Y17-R] ICC/IF image

    Immunocytochemistry: HEK293 cells stained with ARG10579 anti-Nephrin antibody [Y17-R] at 1:700 dilution.

  • ARG10579 anti-Nephrin antibody [Y17-R] WB image

    Western blot: 1) 50 ng, 2) 100 ng, and 3) 200 ng of recombinant Mouse nephrin (extracellular domain, Gln37 - Thr1049) stained with ARG10579 anti-Nephrin antibody [Y17-R].