ARG40667

anti-Nrf 2 phospho (Ser40) antibody

anti-Nrf 2 phospho (Ser40) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Flow cytometry and Human

Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; NRF-2 Nuclear Localization antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Nrf 2 phospho (Ser40)
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Nrf 2
Antigen Species Human
Immunogen Phosphospecific peptide around Ser40 of Human Nrf 2.
Conjugation Un-conjugated
Alternate Names Nuclear factor, erythroid derived 2, like 2; NF-E2-related factor 2; Nuclear factor erythroid 2-related factor 2; HEBP1; NRF2; NFE2-related factor 2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:1000 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4780 Human NFE2L2

Swiss-port # Q16236 Human Nuclear factor erythroid 2-related factor 2

Gene Symbol NFE2L2
Gene Full Name nuclear factor, erythroid 2-like 2
Background This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015]
Function Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. [UniProt]
Cellular Localization Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents. [UniProt]
Highlight Related products:
Nrf 2 antibodies; Nrf 2 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Keap1-Nrf2-ARE antibody panel is launched
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; NRF-2 Nuclear Localization antibody
Calculated MW 68 kDa
PTM Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.

Acetylation at Lys-596 and Lys-599 increases nuclear localization whereas deacetylation by SIRT1 enhances cytoplasmic presence.

Ubiquitinated by the KEAP1-CUL3-RBX1 E3 ubiquitin ligase complex and subject to proteasomal degradation. Ubiquitination is inhibited by sulforaphane. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG40667 anti-Nrf 2 phospho (Ser40) antibody ICC/IF image

    Immunofluorescence: HepG2 cells stained with ARG40667 anti-Nrf 2 phospho (Ser40) antibody.

  • ARG40667 anti-Nrf 2 phospho (Ser40) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma stained with ARG40667 anti-Nrf 2 phospho (Ser40) antibody.

  • ARG40667 anti-Nrf 2 phospho (Ser40) antibody WB image

    Western blot: HepG2 cell lysate stained with ARG40667 anti-Nrf 2 phospho (Ser40) antibody.